May 28, 2022
bioinfogenome

Conditional reprogramming: subsequent technology cellculture

Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian CellCultures

 

Organic organisms are continuously uncovered to an immense repertoire of molecules that cowl environmental or food-derived molecules and medicines, triggering a steady movement of stimuli-dependent diversifications.

  • The variety of those chemical substances in addition to their concentrations contribute to the multiplicity of induced results, together with activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, because the foremost phenotype and manifestation of life, has confirmed to be immensely delicate and extremely adaptive to chemical stimuli.
  • Subsequently, learning the impact of endo- or xenobiotics over mobile metabolism delivers priceless information to apprehend potential mobile exercise of particular person molecules and consider their acute or power advantages and toxicity.
  • The event of contemporary metabolomics applied sciences akin to mass spectrometry or nuclear magnetic resonance spectroscopy now gives unprecedented options for the speedy and environment friendly willpower of metabolic profiles of cells and extra complicated organic methods. Mixed with the supply of well-established cell tradition strategies, these analytical strategies seem completely suited to find out the organic exercise and estimate the constructive and adverse results of chemical substances in quite a lot of cell sorts and fashions, even at hardly detectable concentrations.
  • Metabolic phenotypes will be estimated from learning intracellular metabolites at homeostasis in vivo, whereas in vitro cell cultures present extra entry to metabolites exchanged with progress media.
  • This text discusses analytical options out there for metabolic phenotyping of cell tradition metabolism in addition to the overall metabolomics workflow appropriate for testing the organic exercise of molecular compounds.
  • We emphasize how metabolic profiling of cell supernatants and intracellular extracts can ship priceless and complementary insights for evaluating the consequences of xenobiotics on mobile metabolism. We word that the ideas and strategies mentioned primarily for xenobiotics publicity are extensively relevant to drug testing on the whole, together with endobiotics that cowl energetic metabolites, vitamins, peptides and proteins, cytokines, hormones, nutritional vitamins, and so on.
bioinfogenome
bioinfogenome

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F001 1 ml
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EverBrite Mounting Medium with DAPI

23002 10mL
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FluoreGuard Mounting Medium (Hard Set)

FMH030 30 ml
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FluoreGuard Mounting Medium (Hard Set)

FMH060 60 ml
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FluoreGuard Mounting Medium (Hard Set)

FMH500 500 ml
EUR 327

FluoreGuard Mounting Medium (Hard Set)

FMH999 1000 ml
EUR 530

Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 60ml

NB300 60 ml
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Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 30ml

NB300-30 30 ml
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Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 125ml

NB300A 125 ml
EUR 543

FluoroQuest™ Mounting Medium with DAPI

20004 50 mL
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EverBrite Hardset Mounting Medium with DAPI

23004 10mL
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abx090690-10ml 10 ml
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abx090691-10ml 10 ml
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99-MED 3 ml
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Description: Mounting media for use in microscopy preparations

FluorLast? Mounting Media

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Buffered Glycerol Mounting Media

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Buffered Glycerol Mounting Media

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Buffered Glycerol Mounting Media

GMM999 1000 ml
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PMT030 30 ml
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Permanent Mounting Media (Aqueous)

PMT999 1000 ml
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20009 50 mL
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PolyMount Mounting Media MaxTag Histo (OKRA00045)

OKRA00045 15 ml
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

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COLIFORM MEDIUM

C03-127-2kg 2kg
EUR 327

COLIFORM MEDIUM

C03-127-500g 500 g
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HLP MEDIUM

H08-107-10kg 10 kg
EUR 2278

HLP MEDIUM

H08-107-2Kg 2 Kg
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HLP MEDIUM

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VALI Medium

450 500 ml
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DC MEDIUM

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EUR 261

SIM MEDIUM

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EUR 107

SOB MEDIUM

S19-124-10kg 10 kg
EUR 965

SOB MEDIUM

S19-124-2kg 2kg
EUR 249

SOB MEDIUM

S19-124-500g 500 g
EUR 104

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 103

Medium 199

C0012-01 RT 500 mL Bottle
EUR 105

NeuroProgenitor Medium

NM42400 125 ml
EUR 304

Heller's Medium

CP014-010 10X1L
EUR 99

Heller's Medium

CP014-500 50L
EUR 126

Hoagland's Medium

CP015-010 10X1L
EUR 99

Hoagland's Medium

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M9CA Medium

SD7025 250g
EUR 71.75

YM Medium

SD7031 250g
EUR 70.45

TYGPN Medium

SD7032 250g
EUR 70.01

M63 Medium

SD7033 250g
EUR 71.75

M9 Medium

SD7024 250g
EUR 71.75

A Medium

DJ1018 100g
EUR 84.8

Fine natural hair brush for mounting sections

PO501 each
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Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

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E05-108-10kg 10 kg
EUR 858

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E05-108-2Kg 2 Kg
EUR 226

EC MEDIUM, MODIFIED

E05-108-500g 500 g
EUR 98

A-1 MEDIUM

A01-116-10kg 10 kg
EUR 1034

A-1 MEDIUM

A01-116-2Kg 2 Kg
EUR 264

A-1 MEDIUM

A01-116-500g 500 g
EUR 108

CARY & BLAIR MEDIUM

C03-131-10kg 10 kg
EUR 2185

CARY & BLAIR MEDIUM

C03-131-2Kg 2 Kg
EUR 514

CARY & BLAIR MEDIUM

C03-131-500g 500 g
EUR 176

FLUID THIOGLYCOLLATE MEDIUM

F06-101-10kg 10 kg
EUR 792

FLUID THIOGLYCOLLATE MEDIUM

F06-101-2kg 2kg
EUR 211

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F06-101-500g 500 g
EUR 94

TERRIFIC BROTH MEDIUM

T20-114-10kg 10 kg
EUR 675

TERRIFIC BROTH MEDIUM

T20-114-2kg 2kg
EUR 186

TERRIFIC BROTH MEDIUM

T20-114-500g 500 g
EUR 87

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-10kg 10 kg
EUR 1364

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-2kg 2kg
EUR 336

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-500g 500 g
EUR 128

2XYT MICROBIAL MEDIUM

X24-102-10kg 10 kg
EUR 614

2XYT MICROBIAL MEDIUM

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M13-121-10kg 10 kg
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MOTILITY TEST MEDIUM

M13-121-2Kg 2 Kg
EUR 371

MOTILITY TEST MEDIUM

M13-121-500g 500 g
EUR 137

REINFORCED CLOSTRIDIUM MEDIUM

R18-105-10kg 10 kg
EUR 1194

REINFORCED CLOSTRIDIUM MEDIUM

R18-105-2kg 2kg
EUR 299

REINFORCED CLOSTRIDIUM MEDIUM

R18-105-500g 500 g
EUR 118

Sepadextran-25 Medium

SD25M-100 100 g
EUR 271

Sepadextran-25 Medium

SD25M-500 500 g
EUR 1036

Sepadextran-50 Medium

SD50M-100 100 g
EUR 344

Sepadextran-50 Medium

SD50M-500 500 g
EUR 1285

Conditional reprogramming: subsequent technology cellculture

Lengthy-term major tradition of mammalian cells has been at all times tough attributable to unavoidable senescence. Standard strategies for producing immortalized cell strains normally require manipulation of genome which ends up in change of vital organic and genetic traits. Not too long ago, conditional reprogramming (CR) emerges as a novel subsequent technology instrument for long-term tradition of major epithelium cells derived from nearly all origins with out alteration of genetic background of major cells.

CR co-cultures major cells with inactivated mouse 3T3-J2 fibroblasts within the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling major cells to accumulate stem-like traits whereas retain their potential to completely differentiate. With only some years’ growth, CR exhibits broad prospects in purposes in diversified areas together with illness modeling, regenerative drugs, drug analysis, drug discovery in addition to precision drugs. This evaluate is thus to comprehensively summarize and assess present progress in understanding mechanism of CR and its broad purposes, highlighting the worth of CR in each primary and translational researches and discussing the challenges confronted with CR.

Results of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a mannequin extremely glycosylated therapeutic protein, in a transgenic rice cell suspension tradition handled with kifunensine, a powerful α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

A media trade was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and including recent sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to provide rice recombinant human BChE (rrBChE). Utilizing a 1.25X-concentrated sugar-free medium along with an 80% lowered working quantity through the media trade led to a complete energetic rrBChE manufacturing degree of 79 ± 2 µg (g FW)-1 or 7.5 ± 0.Four mg L-1 within the presence of kifunensine, which was 1.5-times greater than our earlier bioreactor runs utilizing regular sugar-free (NB-S) media with no kifunensine remedy.

Importantly, the quantity of secreted energetic rrBChE in tradition medium was enhanced within the presence of kifunensine, comprising 44% of the entire energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine remedy, which was attributed to completely different N-glycoforms. N-Glycosylation evaluation confirmed considerably elevated oligomannose glycans (Man5/6/7/8) in rrBChE handled with kifunensine in comparison with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the main purpose for incomplete inhibition of α-mannosidase I on this bioreactor examine.

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Bouin's Fluid

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Bouin's Fluid

BNF500 500 ml
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Bouin's Fluid

BNF999 1000 ml
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P-Selectin (PF377), RNA Aptamer, unlabeled

AR-298-U Custom Ask for price

Rat Neuromedin U control/blocking peptide # 2

NMU51-P 100 ug
EUR 164

Human Neuromedin U control/blocking peptide # 3

NMU61-P 100 ug
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Mouse Neuromedin U control/blocking peptide # 4

NMU71-P 100 ug
EUR 164

Michel's Transport Fluid

MTF-10000 10 L
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Michel's Transport Fluid

MTF-20000 20 L
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Michel's Transport Fluid

MTF500 500 ml
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Michel's Transport Fluid

MTF999 1000 ml
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Ansamitocin P-3

ADC-P-003 unit Ask for price

Neuromedin U (R/M/H) control/blocking peptides

NMU41-P 100 ug
EUR 164

AXYPREP MAG PLASMID KIT- MEDIUM - 384 PREPS

MAG-P-M 1/pk
EUR 664
Description: Bioscience Mag Beads; Magnetic Plasmid

HE4, Human ascites fluid

P1438-100
EUR 620

HE4, Human ascites fluid

P1438-500
EUR 2839

SOF Synthetic Oviduct Fluid.

IVL05-100ML 100 ml
EUR 78
Description: Synthetic Oviduct Fluid.

SOF Synthetic Oviduct Fluid.

IVL05-6X100ML 6 x 100 ml
EUR 151
Description: Synthetic Oviduct Fluid.

96-Well, pureGrade, Clear Transparent, U-Bottom, 5 Plates/Sleeve, 100/Unit

33-572X 100 plates
EUR 240
Description:
  • Clear for colorimetric assays
  • Non-treated "medium binding" surface
  • 100% high quality polystyrene
  • 330µl well volume
  • Non-sterile
  • 1 lid included in each stack (20 total lids)
  • 20 stacks of 5 plates, pack of 100

Transparent, 96-Well Plates, pureGrade Sterile, Clear U-Bottom 50 Plates/Unit

91-420U 50 plates
EUR 230
Description:
  • Non-treated medium binding surface
  • 100% high quality polystyrene
  • Individually-wrapped plates w/ lids
  • Sterilized via beta-radiation
  • Available in clear, white or black
  • Free from endotoxins, DNase, DNA, RNase; noncytotoxic

Calci-Clear

NAT1312 EACH
EUR 78

Calci-Clear

NAT1314 EACH
EUR 128

Calci-Clear

NAT1316 EACH
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Histo-Clear

NAT1330 1 US gallon
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Histo-Clear

NAT1332 5 US gallons
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Hepatitis B Virus (HBV) Polymerase (P protein) (A9), RNA Aptamer, unlabeled

AR-233-U Custom Ask for price

Mouse p/CIP/p300 Control/blocking peptide # 1

CIP11-P 100 ug
EUR 164

Mouse p/CIP/p300 Control/blocking peptide # 2

CIP12-P 100 ug
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YLKKIKNSL, P. falciparum circumsporozoite (CSP 334-342) polypeptide

CSPF15-P 1 mg
EUR 286

MICROPLATE, 96 WELL, POLYSTYRENE, CLEAR, ROUND BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, BULK

3797 25/pk
EUR 179
Description: Assay; EIA/RIA - 96 Well

CA-125, Human Ascites Fluid

P1446-10
EUR 294

Human Substance P Peptide (amide salt)

SP15-P-1 1 mg
EUR 164

Human Substance P Peptide (amide salt)

SP15-P-25 25 mg
EUR 529

Human Substance P Peptide (amide salt)

SP15-P-5 5 mg
EUR 286

LEYYLREKAKMAGTLIIPES, P. vivax PvMSP-1 peptide 19 (378-397)

MSPV11-P 1 mg
EUR 164

SKDQIKKLTSLKNKLERRQN, P. vivax PvMSP-1 peptide 53 (1058-1077)

MSPV12-P 1 mg
EUR 164

NFVGKFLELQIPGHTDLLHL, P. vivax PvMSP-1 peptide 4 (78-97)

MSPV13-P 1 mg
EUR 164

FNQLMHVINFHYDLLRANVH, P. vivax PvMSP-1 peptide 6 (118-137)

MSPV14-P 1 mg
EUR 164

LDMLKKVVLGLWKPLDNIKD, P. vivax PvMSP-1 peptide 8 (158-177)

MSPV15-P 1 mg
EUR 164

U-104

9631-25
EUR 414

U-104

9631-5
EUR 142

U-18666A

9469-100 Ask for price

U-18666A

9469-25
EUR 457

U-18666A

9469-5
EUR 153

Kazinol U

TBW00590 unit Ask for price

Gomisin U

TBW01615 5mg Ask for price

Ixerin U

TBZ1502 unit Ask for price

U-93631

B1851-25
EUR 544

U-93631

B1851-5
EUR 175

U-73122

B1887-25
EUR 544

U-73122

B1887-5
EUR 175

U-73343

B1888-5
EUR 175

U-74389G

20-abx076631
  • EUR 314.00
  • EUR 857.00
  • 100 mg
  • 500 mg

U-0126

20-abx076796
  • EUR 1121.00
  • EUR 384.00
  • 25 mg
  • 5 mg

U-101017

HY-19250 1mg
EUR 1125

U-73343

HY-108630 5mg
EUR 234

U-73122

HY-13419 10mM/1mL
EUR 147

U-104

HY-13513 10mM/1mL
EUR 113

U 0126

E1B1901 1mg
EUR 161

U-44069

C5324-1 1 mg
EUR 224
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U-44069

C5324-10 10 mg
EUR 1442
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U-44069

C5324-5 5 mg
EUR 833
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U 90042

B7164-10 10 mg
EUR 350

U 90042

B7164-50 50 mg
EUR 1266

U 89843A

B7165-10 10 mg
EUR 292

U 89843A

B7165-50 50 mg
EUR 1054

U 93631

B7170-10 10 mg
EUR 248

U 93631

B7170-100 100 mg
EUR 1407

U 93631

B7170-5 5 mg
EUR 195

U 93631

B7170-5.1 10 mM (in 1mL DMSO)
EUR 209

U 93631

B7170-50 50 mg
EUR 864

U 73343

B9013-10 10 mg
EUR 224

U 73343

B9013-25 25 mg
EUR 441

U 73343

B9013-5 5 mg
EUR 154

U-104

A4358-10 10 mg
EUR 108
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-25 25 mg
EUR 171
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-5.1 10 mM (in 1mL DMSO)
EUR 108
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-50 50 mg
EUR 270
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-73122

B3422-10 10 mg
EUR 161
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-25 25 mg
EUR 258
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-5 5 mg
EUR 123
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-5.1 10 mM (in 1mL DMSO)
EUR 166
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U 18666A

B6812-10 10 mg
EUR 150
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 18666A

B6812-25 25 mg
EUR 287
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 18666A

B6812-50 50 mg
EUR 473
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 46619

B6890-1 1 mg
EUR 147
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

U 46619

B6890-10 10 mg
EUR 830
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

U 46619

B6890-5 5 mg
EUR 487
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

EC MEDIUM

E05-100-10kg 10 kg
EUR 814

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 216

EC MEDIUM

E05-100-500g 500 g
EUR 95

COLIFORM MEDIUM

C03-127-10kg 10 kg
EUR 1324

COLIFORM MEDIUM

C03-127-2kg 2kg
EUR 327

COLIFORM MEDIUM

C03-127-500g 500 g
EUR 125

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2278

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 534

HLP MEDIUM

H08-107-500g 500 g
EUR 182

VALI Medium

450 500 ml
EUR 265

DC MEDIUM

D04-117-10kg 10 kg
EUR 1579

DC MEDIUM

D04-117-2kg 2kg
EUR 382

DC MEDIUM

D04-117-500g 500 g
EUR 140

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1021

SIM MEDIUM

S19-110-2kg 2kg
EUR 261

SIM MEDIUM

S19-110-500g 500 g
EUR 107

SOB MEDIUM

S19-124-10kg 10 kg
EUR 965

SOB MEDIUM

S19-124-2kg 2kg
EUR 249

SOB MEDIUM

S19-124-500g 500 g
EUR 104

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 103

Medium 199

C0012-01 RT 500 mL Bottle
EUR 105

NeuroProgenitor Medium

NM42400 125 ml
EUR 304

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