Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian CellCultures

 

Organic organisms are continuously uncovered to an immense repertoire of molecules that cowl environmental or food-derived molecules and medicines, triggering a steady movement of stimuli-dependent diversifications.

• The variety of those chemical substances in addition to their concentrations contribute to the multiplicity of induced results, together with activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, because the foremost phenotype and manifestation of life, has confirmed to be immensely delicate and extremely adaptive to chemical stimuli.

• Subsequently, learning the impact of endo- or xenobiotics over mobile metabolism delivers priceless information to apprehend potential mobile exercise of particular person molecules and consider their acute or power advantages and toxicity.

• The event of contemporary metabolomics applied sciences akin to mass spectrometry or nuclear magnetic resonance spectroscopy now gives unprecedented options for the speedy and environment friendly willpower of metabolic profiles of cells and extra complicated organic methods. Mixed with the supply of well-established cell tradition strategies, these analytical strategies seem completely suited to find out the organic exercise and estimate the constructive and adverse results of chemical substances in quite a lot of cell sorts and fashions, even at hardly detectable concentrations.

• Metabolic phenotypes will be estimated from learning intracellular metabolites at homeostasis in vivo, whereas in vitro cell cultures present extra entry to metabolites exchanged with progress media.

• This text discusses analytical options out there for metabolic phenotyping of cell tradition metabolism in addition to the overall metabolomics workflow appropriate for testing the organic exercise of molecular compounds.

• We emphasize how metabolic profiling of cell supernatants and intracellular extracts can ship priceless and complementary insights for evaluating the consequences of xenobiotics on mobile metabolism. We word that the ideas and strategies mentioned primarily for xenobiotics publicity are extensively relevant to drug testing on the whole, together with endobiotics that cowl energetic metabolites, vitamins, peptides and proteins, cytokines, hormones, nutritional vitamins, and so on.

Fluorguard Antifade Mounting Medium
F001	Insitus Biotechnologies	1 ml	EUR 103.2

FluoreGuard Mounting Medium (Hard Set)
FMH030	ScyTek Laboratories	30 ml	EUR 115.2

FluoreGuard Mounting Medium (Hard Set)
FMH060	ScyTek Laboratories	60 ml	EUR 139.2

FluoreGuard Mounting Medium (Hard Set)
FMH500	ScyTek Laboratories	500 ml	EUR 392.4

FluoreGuard Mounting Medium (Hard Set)
FMH999	ScyTek Laboratories	1000 ml	EUR 636

Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 60ml
NB300	Innovex	60 ml	EUR 512.4

Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 30ml
NB300-30	Innovex	30 ml	EUR 331.2

Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 125ml
NB300A	Innovex	125 ml	EUR 651.6

FluoroQuest™ Mounting Medium with DAPI
20004	AAT Bioquest	50 mL	EUR 158.4

EverBrite Hardset Mounting Medium with DAPI
23004	Biotium	10mL	EUR 255.6
Description: Minimum order quantity: 1 unit of 10mL

Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml	Abbexa	10 ml	EUR 243.6

Anti-Fade Mounting Medium (With PI)
abx090691-10ml	Abbexa	10 ml	EUR 243.6

FluoroQuest™ Anti-fading Mounting Medium with DAPI
20005	AAT Bioquest	20 mL	EUR 211

Elix½ Advantage 3
ZRXV003T0	Scientific Laboratory Supplies	EACH	EUR 6914.1

Elix½ Advantage 5
ZRXV005T0	Scientific Laboratory Supplies	EACH	EUR 8211.42

Elix½ Advantage 10
ZRXV010T0	Scientific Laboratory Supplies	EACH	EUR 9118.86

Elix½ Advantage 15
ZRXV015T0	Scientific Laboratory Supplies	EACH	EUR 9876.96

Mounting Media
99-MED	Fitzgerald	3 ml	EUR 253.2
Description: Mounting media for use in microscopy preparations

Milli-Q½ Advantage A10
Z00Q0V0T0	Scientific Laboratory Supplies	EACH	EUR 9451.74

ELIX ADVANTAGE UPGRADE KIT
ZRXSUP0D1	Scientific Laboratory Supplies	EACH	EUR 1837.68

FluorLast? Mounting Media
1213-20	Biovision		EUR 170.4

MILLIPAK MOUNTING KITT
MPKMNTKIT	Scientific Laboratory Supplies	EACH	EUR 189.24

Wall Mounting Kit
WAT2452	Scientific Laboratory Supplies	EACH	EUR 123.12

Wall Mounting Kit
WAT2456	Scientific Laboratory Supplies	EACH	EUR 229.14

WALL MOUNTING BRACKET
WMBSMT002	Scientific Laboratory Supplies	EACH	EUR 157.32

BD 2 ml advantage pipet
356507	Scientific Laboratory Supplies	PK1000	EUR 230.28

BD 25 ml advantage pipette
356535	Scientific Laboratory Supplies	PK200	EUR 159.6

Buffered Glycerol Mounting Media
GMM003	ScyTek Laboratories	3 ml	EUR 73.2

Buffered Glycerol Mounting Media
GMM030	ScyTek Laboratories	30 ml	EUR 86.4

Buffered Glycerol Mounting Media
GMM500	ScyTek Laboratories	500 ml	EUR 219.6

Buffered Glycerol Mounting Media
GMM999	ScyTek Laboratories	1000 ml	EUR 312

Permanent Mounting Media (Aqueous)
PMT030	ScyTek Laboratories	30 ml	EUR 130.8

Permanent Mounting Media (Aqueous)
PMT999	ScyTek Laboratories	1000 ml	EUR 910.8

OPTICAP WALL MOUNTING BRACKET
M0PMNTKIT	Scientific Laboratory Supplies	EACH	EUR 149.34

WALL MOUNTING BRACKET (1)
SYSTFIX01	Scientific Laboratory Supplies	EACH	EUR 287.28

WALL MOUNTING BRACKET TANKPE100
TANKFIXS1	Scientific Laboratory Supplies	EACH	EUR 587.1

Wall Mounting Kit (TANK30)
WAT2448	Scientific Laboratory Supplies	EACH	EUR 115.14

Wall Mounting Kit (TANK60)
WAT2450	Scientific Laboratory Supplies	EACH	EUR 119.7

Elga Wall Mounting Kit
WAT4044	Scientific Laboratory Supplies	EACH	EUR 335.16

Elga Wall Mounting Kit
WAT4046	Scientific Laboratory Supplies	EACH	EUR 132.24

BD 1 ml 1100 advantage pipet
356521	Scientific Laboratory Supplies	PK1000	EUR 202.92

BD Advantage 25ml Serological Pipette Plugged
356525	Scientific Laboratory Supplies	PK200	EUR 161.88

BD Advantage 5ml Serological Pipette Plugged
356543	Scientific Laboratory Supplies	PK200	EUR 78.66

BD Advantage 50ml Serological Pipette Plugged
356550	Scientific Laboratory Supplies	PK100	EUR 173.28

BD Advantage 10ml Serological Pipette Plugged
356551	Scientific Laboratory Supplies	PK200	EUR 77.52

ReadiUse™ microscope mounting solution
20009	AAT Bioquest	50 mL	EUR 138

Mounting Bracket For Base MIC2510
MIC2510	Scientific Laboratory Supplies	EACH	EUR 79.8

WALL MOUNTING BRACKET (1/P)
TANKFIX01	Scientific Laboratory Supplies	EACH	EUR 178.98

Elga Flex Wall Mounting Kit
WAT4170	Scientific Laboratory Supplies	EACH	EUR 406.98

WALL MOUNTING BRACKET W VALVE
WMBSMT001	Scientific Laboratory Supplies	EACH	EUR 207.48

MicroRT room temperature mounting kit
RT-T1	MiTeGen	1 KIT	EUR 64
Description: MicroRT room temperature mounting kit20 MicroRT tubes, razor blade and grease tube

PolyMount Mounting Media MaxTag Histo (OKRA00045)
OKRA00045	Aviva Systems Biology	15 ml	EUR 151.2
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

KL 300 LED Column Mounting Bracket
MIC2528	Scientific Laboratory Supplies	EACH	EUR 58.14

Holder for water tank for mounting
CHA4068	Scientific Laboratory Supplies	EACH	EUR 108.3

WALL MOUNTING BRACKET WASHER PUMP KIT
WMBWASH1	Scientific Laboratory Supplies	EACH	EUR 137.94

Fine natural hair brush for mounting sections
PO501	FD Neurotechnologies	each	EUR 80.4

DC MEDIUM
D04-117-10kg	Alphabiosciences	10 kg	EUR 1894.8

DC MEDIUM
D04-117-2kg	Alphabiosciences	2kg	EUR 458.4

DC MEDIUM
D04-117-500g	Alphabiosciences	500 g	EUR 168

Advanced Medium
C0003-04	Addexbio	RT 500 mL Bottle	EUR 123.6

Medium 199
C0012-01	Addexbio	RT 500 mL Bottle	EUR 126

COLIFORM MEDIUM
C03-127-10kg	Alphabiosciences	10 kg	EUR 1588.8

COLIFORM MEDIUM
C03-127-2kg	Alphabiosciences	2kg	EUR 392.4

COLIFORM MEDIUM
C03-127-500g	Alphabiosciences	500 g	EUR 150

Heller's Medium
CP014-010	GenDepot	10X1L	EUR 118.8

Heller's Medium
CP014-500	GenDepot	50L	EUR 151.2

Hoagland's Medium
CP015-010	GenDepot	10X1L	EUR 118.8

Hoagland's Medium
CP015-500	GenDepot	50L	EUR 151.2

A Medium
DJ1018	Bio Basic	100g	EUR 101.76

EC MEDIUM
E05-100-10kg	Alphabiosciences	10 kg	EUR 976.8

EC MEDIUM
E05-100-2Kg	Alphabiosciences	2 Kg	EUR 259.2

EC MEDIUM
E05-100-500g	Alphabiosciences	500 g	EUR 114

NeuroProgenitor Medium
NM42400	Neuromics	125 ml	EUR 364.8

HLP MEDIUM
H08-107-10kg	Alphabiosciences	10 kg	EUR 2733.6

HLP MEDIUM
H08-107-2Kg	Alphabiosciences	2 Kg	EUR 640.8

HLP MEDIUM
H08-107-500g	Alphabiosciences	500 g	EUR 218.4

M9 Medium
SD7024	Bio Basic	250g	EUR 86.1

M9CA Medium
SD7025	Bio Basic	250g	EUR 86.1

YM Medium
SD7031	Bio Basic	250g	EUR 84.54

TYGPN Medium
SD7032	Bio Basic	250g	EUR 84.01

M63 Medium
SD7033	Bio Basic	250g	EUR 86.1

SIM MEDIUM
S19-110-10kg	Alphabiosciences	10 kg	EUR 1225.2

SIM MEDIUM
S19-110-2kg	Alphabiosciences	2kg	EUR 313.2

SIM MEDIUM
S19-110-500g	Alphabiosciences	500 g	EUR 128.4

SOB MEDIUM
S19-124-10kg	Alphabiosciences	10 kg	EUR 1158

SOB MEDIUM
S19-124-2kg	Alphabiosciences	2kg	EUR 298.8

SOB MEDIUM
S19-124-500g	Alphabiosciences	500 g	EUR 124.8

TCBS Medium
MED1188	Scientific Laboratory Supplies	PK10	EUR 7.52

DNase Medium
MED1196	Scientific Laboratory Supplies	PK10	EUR 7.98

OF Medium
MED1664	Scientific Laboratory Supplies	500G	EUR 68.4

MSC Advantage 1.2 230V 50Hz EN Certified NF listed
CAB2662	Scientific Laboratory Supplies	EACH	EUR 11913

MSC Advantage 1.8 230V 50Hz EN Certified NF listed
CAB2664	Scientific Laboratory Supplies	EACH	EUR 14348.04

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001	Allele Biotech	1 liter	Ask for price

Conditional reprogramming: subsequent technology cellculture

Lengthy-term major tradition of mammalian cells has been at all times tough attributable to unavoidable senescence. Standard strategies for producing immortalized cell strains normally require manipulation of genome which ends up in change of vital organic and genetic traits. Not too long ago, conditional reprogramming (CR) emerges as a novel subsequent technology instrument for long-term tradition of major epithelium cells derived from nearly all origins with out alteration of genetic background of major cells.

CR co-cultures major cells with inactivated mouse 3T3-J2 fibroblasts within the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling major cells to accumulate stem-like traits whereas retain their potential to completely differentiate. With only some years’ growth, CR exhibits broad prospects in purposes in diversified areas together with illness modeling, regenerative drugs, drug analysis, drug discovery in addition to precision drugs. This evaluate is thus to comprehensively summarize and assess present progress in understanding mechanism of CR and its broad purposes, highlighting the worth of CR in each primary and translational researches and discussing the challenges confronted with CR.

Results of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a mannequin extremely glycosylated therapeutic protein, in a transgenic rice cell suspension tradition handled with kifunensine, a powerful α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

A media trade was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and including recent sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to provide rice recombinant human BChE (rrBChE). Utilizing a 1.25X-concentrated sugar-free medium along with an 80% lowered working quantity through the media trade led to a complete energetic rrBChE manufacturing degree of 79 ± 2 µg (g FW)^-1 or 7.5 ± 0.Four mg L^-1 within the presence of kifunensine, which was 1.5-times greater than our earlier bioreactor runs utilizing regular sugar-free (NB-S) media with no kifunensine remedy.

Importantly, the quantity of secreted energetic rrBChE in tradition medium was enhanced within the presence of kifunensine, comprising 44% of the entire energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine remedy, which was attributed to completely different N-glycoforms. N-Glycosylation evaluation confirmed considerably elevated oligomannose glycans (Man5/6/7/8) in rrBChE handled with kifunensine in comparison with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the main purpose for incomplete inhibition of α-mannosidase I on this bioreactor examine.

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
P-2002	EpiGentek	EUR 1084.86 EUR 312.40 EUR 500.50 EUR 738.10	96 Reactions 24 Reactions 48 Reactions 96 Reactions

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit
P-2003	EpiGentek	EUR 1084.86 EUR 312.40 EUR 500.50 EUR 738.10	96 Reactions 24 Reactions 48 Reactions 96 Reactions

Methylamp DNA Modification Kit
P-1001	EpiGentek	EUR 416.70 EUR 149.60 EUR 259.60	80 Reactions 40 Reactions 80 Reactions

FitAmp General Tissue Section DNA Isolation Kit
P-1003	EpiGentek	EUR 754.26 EUR 253.00 EUR 490.60	100 Samples 50 Samples 100 Samples

FitAmp Plasma/Serum DNA Isolation Kit
P-1004	EpiGentek	EUR 416.70 EUR 149.60 EUR 253.00	100 Samples 50 Samples 100 Samples

TuMinute PCR Clean-Up Kit
P-1005	EpiGentek	EUR 303.60 EUR 90.20 EUR 171.60	100 Samples 50 Samples 100 Samples

DNA Concentrator Kit
P-1006	EpiGentek	EUR 303.60 EUR 90.20 EUR 171.60	100 Samples 50 Samples 100 Samples

FitAmp Paraffin Tissue Section DNA Isolation Kit
P-1009	EpiGentek	EUR 702.06 EUR 239.80 EUR 454.30	100 Samples 50 Samples 100 Samples

Methylamp One-Step DNA Modification Kit
P-1010	EpiGentek	EUR 416.70 EUR 148.50 EUR 253.00	80 Samples 40 Samples 80 Samples

Methylamp Universal Methylated DNA Kit
P-1011	EpiGentek	EUR 684.66 EUR 292.60 EUR 442.20	20 Modifications 10 Modifications 20 Modifications

FitAmp Circulating DNA Quantification Kit
P-1012	EpiGentek	EUR 595.92 EUR 217.80 EUR 387.20	96 Assays 48 Assays 96 Assays

Methylamp Global DNA Methylation Quantification Ultra Kit
P-1014B	EpiGentek	EUR 1137.06 EUR 429.00 EUR 760.10	96 Assays 48 Assays 96 Assays

Methylamp Methylated DNA Capture (MeDIP) Kit
P-1015	EpiGentek	EUR 1050.06 EUR 424.60 EUR 712.80	48 Reactions 24 Reactions 48 Reactions

Methylamp Whole Cell Bisulfite Modification Kit
P-1016	EpiGentek	EUR 595.92 EUR 213.40 EUR 380.60	80 Samples 40 Samples 80 Samples

FitAmp Urine DNA Isolation Kit
P-1017	EpiGentek	EUR 474.12 EUR 171.60 EUR 292.60	100 Samples 50 Samples 100 Samples

FitAmp Blood and Cultured Cell DNA Extraction Kit
P-1018	EpiGentek	EUR 468.90 EUR 166.10 EUR 294.80	100 Samples 50 Samples 100 Samples

FitAmp General DNA Quantification Kit
P-1020	EpiGentek	EUR 361.02 EUR 126.50 EUR 213.40	96 Assays 48 Assays 96 Assays

SuperSense Methylated DNA Quantification Kit
P-1021	EpiGentek	EUR 1137.06 EUR 442.20 EUR 760.10	96 Assays 48 Assays 96 Assays

Methylamp MS-qPCR Fast Kit
P-1028	EpiGentek	EUR 595.92 EUR 213.40 EUR 380.60	200 Reactions 100 Reactions 200 Reactions

EpiQuik Quantitative PCR Fast Kit
P-1029	EpiGentek	EUR 595.92 EUR 213.40 EUR 380.60	200 Reactions 100 Reactions 200 Reactions

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
P-1032	EpiGentek	EUR 1067.46 EUR 396.00 EUR 696.30	96 reactions 48 reactions 96 reactions

MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
P-1034	EpiGentek	EUR 1050.06 EUR 387.20 EUR 712.80	96 Assays 48 Assays 96 Assays

MethylFlash Methylated DNA Quantification Kit (Fluorometric)
P-1035	EpiGentek	EUR 1084.86 EUR 424.60 EUR 738.10	96 Assays 48 Assays 96 Assays

MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)
P-1036	EpiGentek	EUR 1084.86 EUR 424.60 EUR 738.10	96 Assays 48 Assays 96 Assays

MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)
P-1037	EpiGentek	EUR 1137.06 EUR 463.10 EUR 775.50	96 Assays 48 Assays 96 Assays

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)
P-1039	EpiGentek	EUR 945.66 EUR 360.80 EUR 625.90	96 Assays 48 Assays 96 Assays

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Fluorometric)
P-1040	EpiGentek	EUR 945.66 EUR 360.80 EUR 625.90	96 Assays 48 Assays 96 Assays

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric)
P-1041	EpiGentek	EUR 1137.06 EUR 442.20 EUR 760.10	96 Assays 48 Assays 96 Assays

EpiNext DNA Library Preparation Kit (Illumina)
P-1051	EpiGentek	EUR 1415.46 EUR 552.20 EUR 955.90	24 Reactions 12 Reactions 24 Reactions

EpiQuik MeDIP Ultra Kit
P-1052	EpiGentek	EUR 1206.66 EUR 466.40 EUR 810.70	48 Reactions 24 Reactions 48 Reactions

EpiNext High-Sensitivity DNA Library Preparation Kit (Illumina)
P-1053	EpiGentek	EUR 1415.46 EUR 552.20 EUR 955.90	24 Reactions 12 Reactions 24 Reactions

EpiNext Post-Bisulfite DNA Library Preparation Kit (Illumina)
P-1055	EpiGentek	EUR 1485.06 EUR 599.50 EUR 1006.50	24 Reactions 12 Reactions 24 Reactions

EpiNext Bisulfite Sequencing Kit (Illumina)
P-1056	EpiGentek	EUR 1798.26 EUR 625.90 EUR 1225.40	24 Reactions 12 Reactions 24 Reactions

EpiNext DNA Size Selection Kit
P-1059	EpiGentek	EUR 361.02 EUR 127.60 EUR 213.40	96 reactions 48 reactions 96 reactions

EpiQuik Circulating Cell-Free DNA Isolation Kit
P-1064	EpiGentek	EUR 416.70 EUR 148.50 EUR 253.00	50 Isolations 25 Isolations 50 Isolations

EpiQuik Circulating Cell-Free DNA (ccfDNA) Isolation Easy Kit
P-1065	EpiGentek	EUR 416.70 EUR 148.50 EUR 253.00	50 Isolations 25 Isolations 50 Isolations

EpiQuik Methyl-Histone H3K9 ChIP Kit
P-2006	EpiGentek	EUR 1050.06 EUR 442.20 EUR 699.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Methyl-Histone H3K4 ChIP Kit
P-2007	EpiGentek	EUR 1032.66 EUR 416.90 EUR 686.40	48 Reactions 24 Reactions 48 Reactions

EpiQuik Tissue Methyl-Histone H3K9 ChIP Kit
P-2008	EpiGentek	EUR 1050.06 EUR 442.20 EUR 699.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Tissue Methyl-Histone H3K4 ChIP Kit
P-2009	EpiGentek	EUR 1050.06 EUR 442.20 EUR 699.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Acetyl-Histone H3 ChIP Kit
P-2010	EpiGentek	EUR 1050.06 EUR 442.20 EUR 699.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Acetyl-Histone H4 ChIP Kit
P-2011	EpiGentek	EUR 1050.06 EUR 442.20 EUR 699.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Tissue Acetyl-Histone H3 ChIP Kit
P-2012	EpiGentek	EUR 1050.06 EUR 442.20 EUR 699.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Tissue Acetyl-Histone H4 ChIP Kit
P-2013	EpiGentek	EUR 1050.06 EUR 442.20 EUR 699.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Plant ChIP Kit
P-2014	EpiGentek	EUR 945.66 EUR 387.20 EUR 639.10	48 Reactions 24 Reactions 48 Reactions

EpiQuik Methyl-Histone H3K27 ChIP Kit
P-2015	EpiGentek	EUR 1067.46 EUR 490.60 EUR 710.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Tissue Methyl-Histone H3K27 ChIP Kit
P-2016	EpiGentek	EUR 1067.46 EUR 490.60 EUR 710.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Methyl-CpG Binding Domain Protein 2 ChIP Kit
P-2017	EpiGentek	EUR 1067.46 EUR 490.60 EUR 710.60	48 Reactions 24 Reactions 48 Reactions

EpiQuik Methylated DNA Immunoprecipitation Kit
P-2019	EpiGentek	EUR 1067.46 EUR 451.00 EUR 724.90	48 Reactions 24 Reactions 48 Reactions

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit
P-2020	EpiGentek	EUR 1067.46 EUR 442.20 EUR 710.60	48 Reactions 24 Reactions 48 Reactions

ChromaFlash One-Step ChIP Kit
P-2025	EpiGentek	EUR 1067.46 EUR 451.00 EUR 724.90	96 reactions 48 reactions 96 reactions

ChromaFlash One-Step Magnetic ChIP Kit
P-2026	EpiGentek	EUR 1067.46 EUR 451.00 EUR 724.90	96 Reactions 48 Reactions 96 Reactions

ChromaFlash High-Sensitivity ChIP Kit
P-2027	EpiGentek	EUR 771.66 EUR 338.80 EUR 512.60	48 Reactions 24 Reactions 48 Reactions

EpiNext ChIP-Seq High-Sensitivity Kit (Illumina)
P-2030	EpiGentek	EUR 1798.26 EUR 712.80 EUR 1249.60	24 Reactions 12 Reactions 24 Reactions

Pre-Sure ChIP Antibody Validation Kit
P-2031	EpiGentek	EUR 1241.46 EUR 503.80 EUR 833.80	96 reactions 48 reactions 96 reactions

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
P-3001	EpiGentek	EUR 1050.06 EUR 424.60 EUR 712.80	96 Assays 48 Assays 96 Assays

EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K4)
P-3002	EpiGentek	EUR 1067.46 EUR 437.80 EUR 724.90	96 Assays 48 Assays 96 Assays

EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K9)
P-3003	EpiGentek	EUR 1067.46 EUR 437.80 EUR 724.90	96 Assays 48 Assays 96 Assays

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric)
P-3004	EpiGentek	EUR 1067.46 EUR 429.00 EUR 710.60	96 Assays 48 Assays 96 Assays

EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K27)
P-3005	EpiGentek	EUR 1067.46 EUR 437.80 EUR 724.90	96 Assays 48 Assays 96 Assays

EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit
P-3006A	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit
P-3007A	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit
P-3008	EpiGentek	EUR 1032.66 EUR 454.30 EUR 686.40	96 Assays 48 Assays 96 Assays

EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)
P-3009	EpiGentek	EUR 1067.46 EUR 437.80 EUR 724.90	96 Assays 48 Assays 96 Assays

EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Fluorometric)
P-3010	EpiGentek	EUR 1067.46 EUR 451.00 EUR 724.90	96 Assays 48 Assays 96 Assays

EpiQuik Dnmt1 Assay Kit
P-3011	EpiGentek	EUR 1067.46 EUR 512.60 EUR 724.90	96 Assays 48 Assays 96 Assays

EpiQuik Dnmt3A Assay Kit
P-3012	EpiGentek	EUR 1067.46 EUR 503.80 EUR 710.60	96 Assays 48 Assays 96 Assays

EpiQuik Dnmt3B Assay Kit
P-3013	EpiGentek	EUR 1067.46 EUR 503.80 EUR 710.60	96 Assays 48 Assays 96 Assays

EpiQuik In Situ Histone H3K27 Tri-Methylation Assay Kit
P-3014T	EpiGentek	EUR 1276.26 EUR 503.80 EUR 858.00	2x96 assays 96 Assays 2x96 assays

EpiQuik In Situ Histone H3K4 Methylation Assay Kit
P-3015	EpiGentek	EUR 1276.26 EUR 503.80 EUR 858.00	2x96 assays 96 Assays 2x96 assays

EpiQuik In Situ Histone H3K9 Methylation Assay Kit
P-3016	EpiGentek	EUR 1276.26 EUR 503.80 EUR 858.00	2x96 assays 96 Assays 2x96 assays

EpiQuik Global Histone H3K4 Methylation Assay Kit
P-3017	EpiGentek	EUR 1067.46 EUR 477.40 EUR 710.60	96 Assays 48 Assays 96 Assays

EpiQuik Global Histone H3K9 Methylation Assay Kit
P-3018	EpiGentek	EUR 1067.46 EUR 477.40 EUR 710.60	96 Assays 48 Assays 96 Assays

EpiQuik DNA Demethylase Activity/Inhibition Assay Kit
P-3019	EpiGentek	EUR 1067.46 EUR 477.40 EUR 710.60	96 Assays 48 Assays 96 Assays

EpiQuik Global Histone H3K27 Methylation Assay Kit
P-3020	EpiGentek	EUR 1067.46 EUR 477.40 EUR 710.60	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K4 Quantification Kit (Colorimetric)
P-3022	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K4 Quantification Kit (Fluorometric)
P-3023	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K4 Quantification Kit (Colorimetric)
P-3024	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K4 Quantification Kit (Fluorometric)
P-3025	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K4 Quantification Kit (Colorimetric)
P-3026	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K4 Quantification Kit (Fluorometric)
P-3027	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K9 Quantification Kit (Colorimetric)
P-3030	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K9 Quantification Kit (Fluorometric)
P-3031	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K9 Quantification Kit (Colorimetric)
P-3032	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K9 Quantification Kit (Fluorometric)
P-3033	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K9 Quantification Kit (Colorimetric)
P-3034	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K9 Quantification Kit (Fluorometric)
P-3035	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K27 Quantification Kit (Colorimetric)
P-3038	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K27 Quantification Kit (Fluorometric)
P-3039	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K27 Quantification Kit (Colorimetric)
P-3040	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K27 Quantification Kit (Fluorometric)
P-3041	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K27 Quantification Kit (Colorimetric)
P-3042	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K27 Quantification Kit (Fluorometric)
P-3043	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K36 Quantification Kit (Colorimetric)
P-3046	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K36 Quantification Kit (Fluorometric)
P-3047	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K36 Quantification Kit (Colorimetric)
P-3048	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K36 Quantification Kit (Fluorometric)
P-3049	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K36 Quantification Kit (Colorimetric)
P-3050	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K36 Quantification Kit (Fluorometric)
P-3051	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K79 Quantification Kit (Colorimetric)
P-3054	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H3K79 Quantification Kit (Fluorometric)
P-3055	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K79 Quantification Kit (Colorimetric)
P-3056	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H3K79 Quantification Kit (Fluorometric)
P-3057	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K79 Quantification Kit (Colorimetric)
P-3058	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H3K79 Quantification Kit (Fluorometric)
P-3059	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Total Histone H3 Quantification Kit (Colorimetric)
P-3062	EpiGentek	EUR 910.86 EUR 330.00 EUR 599.50	96 Assays 48 Assays 96 Assays

EpiQuik Total Histone H3 Quantification Kit (Fluorometric)
P-3063	EpiGentek	EUR 945.66 EUR 333.30 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H4K20 Quantification Kit (Colorimetric)
P-3064	EpiGentek	EUR 945.66 EUR 347.60 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Mono-Methyl Histone H4K20 Quantification Kit (Fluorometric)
P-3065	EpiGentek	EUR 945.66 EUR 347.60 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H4K20 Quantification Kit (Colorimetric)
P-3066	EpiGentek	EUR 945.66 EUR 347.60 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Di-Methyl Histone H4K20 Quantification Kit (Fluorometric)
P-3067	EpiGentek	EUR 945.66 EUR 347.60 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H4K20 Quantification Kit (Colorimetric)
P-3068	EpiGentek	EUR 945.66 EUR 347.60 EUR 625.90	96 Assays 48 Assays 96 Assays

EpiQuik Global Tri-Methyl Histone H4K20 Quantification Kit (Fluorometric)
P-3069	EpiGentek	EUR 945.66 EUR 347.60 EUR 625.90	96 Assays 48 Assays 96 Assays