September 13, 2026
bioinfogenome

Conditional reprogramming: subsequent technology cellculture

Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian CellCultures

 

Organic organisms are continuously uncovered to an immense repertoire of molecules that cowl environmental or food-derived molecules and medicines, triggering a steady movement of stimuli-dependent diversifications.

  • The variety of those chemical substances in addition to their concentrations contribute to the multiplicity of induced results, together with activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, because the foremost phenotype and manifestation of life, has confirmed to be immensely delicate and extremely adaptive to chemical stimuli.
  • Subsequently, learning the impact of endo- or xenobiotics over mobile metabolism delivers priceless information to apprehend potential mobile exercise of particular person molecules and consider their acute or power advantages and toxicity.
  • The event of contemporary metabolomics applied sciences akin to mass spectrometry or nuclear magnetic resonance spectroscopy now gives unprecedented options for the speedy and environment friendly willpower of metabolic profiles of cells and extra complicated organic methods. Mixed with the supply of well-established cell tradition strategies, these analytical strategies seem completely suited to find out the organic exercise and estimate the constructive and adverse results of chemical substances in quite a lot of cell sorts and fashions, even at hardly detectable concentrations.
  • Metabolic phenotypes will be estimated from learning intracellular metabolites at homeostasis in vivo, whereas in vitro cell cultures present extra entry to metabolites exchanged with progress media.
  • This text discusses analytical options out there for metabolic phenotyping of cell tradition metabolism in addition to the overall metabolomics workflow appropriate for testing the organic exercise of molecular compounds.
  • We emphasize how metabolic profiling of cell supernatants and intracellular extracts can ship priceless and complementary insights for evaluating the consequences of xenobiotics on mobile metabolism. We word that the ideas and strategies mentioned primarily for xenobiotics publicity are extensively relevant to drug testing on the whole, together with endobiotics that cowl energetic metabolites, vitamins, peptides and proteins, cytokines, hormones, nutritional vitamins, and so on.
bioinfogenome
bioinfogenome

Mounting Medium

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Pertex Mounting Medium

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Pertex Mounting Medium

RRSP008-11 500ml
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Kisser's Mounting Medium

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Acrytol Mounting Medium

21730018-1 100 mL
EUR 94.01
Description: Methylbenzene, methylbenzol, phenylmethane, toluol, methacrylic acid methyl ester, MMA

Aqueous Mounting Medium

AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 96

Antifade Mounting Medium

AR1109 10mL (for 1000 assays)
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Glycerol mounting medium

AR-6508-01 30 ml
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Glycerol mounting medium

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SuperMOUNT? Mounting Medium

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PT Mount - Mounting Medium

PTM100 100 ml
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PT Mount - Mounting Medium

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FluoreGuard Mounting Medium

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FluoreGuard Mounting Medium

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FluoreGuard Mounting Medium

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Anti-Fade Mounting Medium

abx090692-100l 100 µl
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Anti-Fade Mounting Medium

abx090692-10ml 10 ml
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Anti-Fade Mounting Medium

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ISH Mounting Medium, 18 ml

E21-18 -
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Mounting Medium, Organic, BioAssay

MBS635281-15mL 15mL
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Mounting Medium, Organic, BioAssay

MBS635281-5x15mL 5x15mL
EUR 1370

PERMANENT AQUEOUS MOUNTING MEDIUM

MBS239099-100mL 100mL
EUR 410

PERMANENT AQUEOUS MOUNTING MEDIUM

MBS239099-5x100mL 5x100mL
EUR 1665

PERMANENT AQUEOUS MOUNTING MEDIUM

MBS239106-30mL 30mL
EUR 250

PERMANENT AQUEOUS MOUNTING MEDIUM

MBS239106-5x30mL 5x30mL
EUR 960

PERMANENT AQUEOUS MOUNTING MEDIUM

MBS239108-250mL 250mL
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PERMANENT AQUEOUS MOUNTING MEDIUM

MBS239108-5x250mL 5x250mL
EUR 3145

FluoroQuestâ„¢ Antifade Mounting Medium

20007 5mL
EUR 112
Description: FluoroQuestâ„¢ Antifade Mounting Medium is a specialized and stable formula that effectively preserves fluorescence by preventing rapid photobleaching of fluorescent proteins and dyes.

FluoroQuestâ„¢ Antifade Mounting Medium

20007-5mL 5 mL
EUR 109
Description: FluoroQuestâ„¢ Antifade Mounting Medium is a specialized and stable formula that effectively preserves fluorescence by preventing rapid photobleaching of fluorescent proteins and dyes.

Antifade Mounting Medium with PI

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Fluorescent Mounting Medium, 18 ml

E18-18 -
EUR 98

Kisser's Mounting Medium (phenol free)

C0183 10 mL Ask for price

Antifade Mounting Medium with DAPI

P0131-25ml 25 mL Ask for price

Antifade Mounting Medium with DAPI

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Glycerol mounting medium with DABCO

AR-6509-01 30 ml
EUR 60.05

Glycerol mounting medium with DABCO

AR-6509-02 100ml
EUR 151.2

FluoreGuard Mounting Medium (Hard Set)

FMH030 30 ml
EUR 37.28

FluoreGuard Mounting Medium (Hard Set)

FMH060 60 ml
EUR 52.72

FluoreGuard Mounting Medium (Hard Set)

FMH500 500 ml
EUR 222.42

FluoreGuard Mounting Medium (Hard Set)

FMH999 1000 ml
EUR 385.71

Mounting Medium, antifading (with DAPI)

S2110
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Fluoromount Mounting Medium with DAPI

42734051-1 20 mL
EUR 98.6

FluoroQuestâ„¢ Mounting Medium with DAPI

20004-50mL 50 mL
EUR 109
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

FluoroQuestâ„¢ Mounting Medium with DAPI

20004 50 mL
EUR 112
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Anti-Fade Mounting Medium (With PI)

abx090691-100l 100 µl
EUR 137.5

Anti-Fade Mounting Medium (With PI)

abx090691-10ml 10 ml
EUR 243.6

Anti-Fade Mounting Medium (With PI)

abx090691-1ml 1 ml Ask for price

Anti-Fade Mounting Medium (With PI)

abx090691-200l 200 µl Ask for price

Anti-fade Mounting Medium (With PI)

MBS355740-10mL 10mL
EUR 180

Anti-fade Mounting Medium (With PI)

MBS355740-5x10mL 5x10mL
EUR 515

REMBRANDT® Fluorescent mounting medium

Z000R.0050 1ml
EUR 16

PERMANENT AQUEOUS MOUNTING MEDIUM Reagent

GWB-Q00293 30 ml Ask for price

PERMANENT AQUEOUS MOUNTING MEDIUM Reagent

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GWB-Q00295 250 ml Ask for price

Anti-Fade Mounting Medium (With DAPI)

abx090690-100l 100 µl
EUR 137.5

Anti-Fade Mounting Medium (With DAPI)

abx090690-10ml 10 ml
EUR 243.6

Anti-Fade Mounting Medium (With DAPI)

abx090690-1ml 1 ml Ask for price

Anti-Fade Mounting Medium (With DAPI)

abx090690-200l 200 µl Ask for price

Anti-fade Mounting Medium (With DAPI)

MBS355738-10mL 10mL
EUR 180

Anti-fade Mounting Medium (With DAPI)

MBS355738-5x10mL 5x10mL
EUR 515

FluoroQuestâ„¢ PLUS Antifade Mounting Medium

20008 5mL
EUR 132
Description: FluoroQuestâ„¢ PLUS Antifade Mounting Medium, the latest breakthrough in non-setting media technology, provides unparalleled protection against fading throughout the visible spectrum, including far-red wavelengths.

FluoroQuestâ„¢ PLUS Antifade Mounting Medium

20008-5mL 5 mL
EUR 129
Description: FluoroQuestâ„¢ PLUS Antifade Mounting Medium, the latest breakthrough in non-setting media technology, provides unparalleled protection against fading throughout the visible spectrum, including far-red wavelengths.

SuperKine Enhanced Antifade Mounting Medium

MBS9719197-10mL 10mL
EUR 100

SuperKine Enhanced Antifade Mounting Medium

MBS9719197-5x10mL 5x10mL
EUR 405

Antifade Mounting Medium with Hoechst 33342

P0133-25ml 25 mL Ask for price

Antifade Mounting Medium with Hoechst 33342

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Antifade Mounting Medium (with Hoechst 33342)

S2111
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Mounting Medium, Antifading (PVP, with DAPI)

S2135 25 mL Ask for price

Enhanced Antifade Mounting Medium with DAPI

MBS9719255-10mL 10mL
EUR 115

Enhanced Antifade Mounting Medium with DAPI

MBS9719255-50mL 50mL
EUR 140

Enhanced Antifade Mounting Medium with DAPI

MBS9719255-5x10mL 5x10mL
EUR 500

Enhanced Antifade Mounting Medium with DAPI

MBS9719255-5x50mL 5x50mL
EUR 615

Fluorescent Mounting Medium with PI, 18 ml

E20-18 -
EUR 165

RSTAIN Fluorguard™ Antifade Mounting Medium

F001 1 ml Ask for price

EverBrite TrueBlack® Hardset Mounting Medium

23017-1 EA
EUR 304

EverBrite TrueBlack® Hardset Mounting Medium

23017-T 1ML
EUR 81
Description: N/A

EverBrite TrueBlack® Hardset Mounting Medium

23017-T-1 EA
EUR 81

SuperKine™ Enhanced Antifade Mounting Medium

BMU104-EN-10mL 10 mL
EUR 39
Description: SuperKine™ Enhanced Antifade Mounting Medium is compatible with a wide range of fluorescent dyes and protects the entire visible and infrared spectrum from fluorescence signal quenching.

SuperKine™ Enhanced Antifade Mounting Medium

BMU104-EN-50mL 50 mL
EUR 69
Description: SuperKine™ Enhanced Antifade Mounting Medium is compatible with a wide range of fluorescent dyes and protects the entire visible and infrared spectrum from fluorescence signal quenching.

SuperKine™ Enhanced Antifade Mounting Medium

BMU104-EN-each each Ask for price

Conditional reprogramming: subsequent technology cellculture

Lengthy-term major tradition of mammalian cells has been at all times tough attributable to unavoidable senescence. Standard strategies for producing immortalized cell strains normally require manipulation of genome which ends up in change of vital organic and genetic traits. Not too long ago, conditional reprogramming (CR) emerges as a novel subsequent technology instrument for long-term tradition of major epithelium cells derived from nearly all origins with out alteration of genetic background of major cells.

CR co-cultures major cells with inactivated mouse 3T3-J2 fibroblasts within the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling major cells to accumulate stem-like traits whereas retain their potential to completely differentiate. With only some years’ growth, CR exhibits broad prospects in purposes in diversified areas together with illness modeling, regenerative drugs, drug analysis, drug discovery in addition to precision drugs. This evaluate is thus to comprehensively summarize and assess present progress in understanding mechanism of CR and its broad purposes, highlighting the worth of CR in each primary and translational researches and discussing the challenges confronted with CR.

Results of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a mannequin extremely glycosylated therapeutic protein, in a transgenic rice cell suspension tradition handled with kifunensine, a powerful α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

A media trade was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and including recent sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to provide rice recombinant human BChE (rrBChE). Utilizing a 1.25X-concentrated sugar-free medium along with an 80% lowered working quantity through the media trade led to a complete energetic rrBChE manufacturing degree of 79 ± 2 µg (g FW)-1 or 7.5 ± 0.Four mg L-1 within the presence of kifunensine, which was 1.5-times greater than our earlier bioreactor runs utilizing regular sugar-free (NB-S) media with no kifunensine remedy.

Importantly, the quantity of secreted energetic rrBChE in tradition medium was enhanced within the presence of kifunensine, comprising 44% of the entire energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine remedy, which was attributed to completely different N-glycoforms. N-Glycosylation evaluation confirmed considerably elevated oligomannose glycans (Man5/6/7/8) in rrBChE handled with kifunensine in comparison with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the main purpose for incomplete inhibition of α-mannosidase I on this bioreactor examine.

FLUID THIOGLYCOLLATE MEDIUM

F06-101-2kg 2kg
EUR 253.2

FLUID THIOGLYCOLLATE MEDIUM

F06-101-500g 500 g
EUR 112.8

Fluid Thioglycollate Medium

RL100110
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Fluid Thioglycollate Medium

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Fluid Thioglycollate Medium

LB2660 500 mL Ask for price

Fluid Thioglycollate Medium Solution

40120793-1 12x100 mL
EUR 244.03

Fluid Thioglycollate Medium, Harmonized

46990 100 Gms
EUR 4.28
Description: Part D

Fluid Thioglycollate Medium, Harmonized

46990-1 500 Gms
EUR 14.54
Description: Part D

Fluid Thioglycollate Medium w/K Agar (Powder)

MBS652904-500g 500g
EUR 290

Fluid Thioglycollate Medium w/K Agar (Powder)

MBS652904-5x500g 5x500g
EUR 1150

Alternative Thioglycollate Medium (I/P)(U/P)

52023-1 500 Gms
EUR 12.83
Description: Part D

Thioglycollate Medium w/o Indicator (Diagnostic Thioglycollate Medium)

91690 100 Gms
EUR 6.84
Description: Part D

Thioglycollate Medium w/o Indicator (Diagnostic Thioglycollate Medium)

91690-1 500 Gms
EUR 22.24
Description: Part D

Brewer Thioglycollate Medium

33355 100 Gms
EUR 5.13
Description: Part D

Brewer Thioglycollate Medium

33355-1 500 Gms
EUR 22.24
Description: Part D

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-10kg 10 kg
EUR 1636.8

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-2kg 2kg
EUR 403.2

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-500g 500 g
EUR 153.6

Thioglycollate Medium (Brewer)

LA4590 250 g Ask for price

Thioglycollate Medium (Granulated)

LB1700 250 g Ask for price

Thioglycollate Broth Medium

LA8740 250 g Ask for price

Thioglycollate Medium (without agar)

LA4190 250 g Ask for price

Alternative Thioglycollate Medium, Sterile

39422 100 Gms
EUR 4.28
Description: Part D

Alternative Thioglycollate Medium, Sterile

39422-1 500 Gms
EUR 18.82
Description: Part D

Thioglycollate Medium w/o Indicator (Powder)

MBS652653-500g 500g
EUR 415

Thioglycollate Medium w/o Indicator (Powder)

MBS652653-5x500g 5x500g
EUR 1710

Thioglycollate Broth w/ Liver Extract (B.Q. Vaccine Medium)

38136 100 Gms
EUR 6.84
Description: Part D

Thioglycollate Broth w/ Liver Extract (B.Q. Vaccine Medium)

38136-1 500 Gms
EUR 18.82
Description: Part D

ALT. THIOGLYCOLLATE MED.

A01-103-10kg 10 kg Ask for price

ALT. THIOGLYCOLLATE MED.

A01-103-2Kg 2 Kg Ask for price

ALT. THIOGLYCOLLATE MED.

A01-103-500g 500 g Ask for price

Thioglycollate Agar

65489 100 Gms
EUR 8.55
Description: Part D

Thioglycollate Agar

65489-1 500 Gms
EUR 34.21
Description: Part D

SODIUM THIOGLYCOLLATE

S19-136-10kg 10 kg Ask for price

SODIUM THIOGLYCOLLATE

S19-136-2Kg 2 Kg Ask for price

SODIUM THIOGLYCOLLATE

S19-136-500g 500 g Ask for price

Thioglycollate U.S.P. - PK50

MED1126 PK50
EUR 62.1

Schaedler Agar w/o Thioglycollate

58249 100 Gms
EUR 12.83
Description: Part D

Schaedler Agar w/o Thioglycollate

58249-1 500 Gms
EUR 37.63
Description: Part D

Sodium Thioglycollate For Bacteriology

1191440 100 100 g Ask for price

Sodium Thioglycollate For Bacteriology

1191440 250 250 g Ask for price

Fluid Thiogly Medium (USP) - EACH

MED1472 EACH
EUR 90.45

Fluid Thio Medium 500gm - EACH

225650 EACH
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LA8520 500 g Ask for price

TPY Fluid Medium

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NBRIP Fluid Medium

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Sauton’s Fluid Medium

LA8760 250 g Ask for price

Fluid Lactose Medium

LA2350 250 g Ask for price

Fluid Sabouraud Medium

93466 100 Gms
EUR 4.28
Description: Part D

Fluid Sabouraud Medium

93466-1 500 Gms
EUR 17.11
Description: Part D

Sabouraud Medium Fluid (Powder)

MBS653274-500g 500g
EUR 275

Sabouraud Medium Fluid (Powder)

MBS653274-5x500g 5x500g
EUR 1085

Fluid Selenite-Cystine Medium

LA2690 250 g Ask for price

Fluid Tetrathionate Medium Base

LA3000 250 g Ask for price

Carbon Source Test Fluid Medium

LA8770 250 g Ask for price

Nitrogen Source Test Fluid Medium

LA8780 250 g Ask for price

Ashby's Fluid Medium for Azotobacter

LB0630 250 g Ask for price

TGC Medium without Indicator , Fluid

LA2080 250 g Ask for price

Uchimiya and Miurashige Fluid Medium

LA8481 500 g Ask for price

MEDIUM BLOT BOX - CLEAR

BLWVS0028 9.1x6.6x2.9CM
EUR 32.42

Thioglycolic acid

GK6903 250ml
EUR 209.98

Thioglycolic acid

GK6903-1 1
EUR 114.6

Thioglycolic acid

GK6903-1L 1 l
EUR 175.2

Thioglycolic acid

GK6903-250 250
EUR 31.7

Thioglycolic acid

GK6903-2500 2500
EUR 229.3

Thioglycolic acid

GK6903-2500ML 2500 ml
EUR 313.2

Thioglycolic acid

GK6903-250ML 250 ml
EUR 74.4

Thioglycolic acid

GK6903-500 500
EUR 59.3

Thioglycolic acid

GK6903-500ML 500 ml
EUR 108

Thioglycolic Acid (~90%)

T350755 100g
EUR 127
Description: 68-11-1

Thioglycolic Acid

T350760 100g
EUR 68
Description: 68-11-1

Thioglycolic acid

TRC-T350760-100G 100 g Ask for price

Thioglycolic Acid

TRC-T350760-10G 10 g Ask for price

Thioglycolic Acid

TRC-T350760-1G 1 g Ask for price

Thioglycolic Acid pure, 80%

35357 500 ml
EUR 12.32
Description: Part A

Octyl thioglycolate

O02270 25G
EUR 79.64
Description: CAS N° 7664-80-4

Sodium Thioglycolate

32003-22 25G
EUR 22.4

Sodium Thioglycolate

40000047-1 25 g
EUR 36.49

Sodium Thioglycolate

40000047-2 100 g
EUR 105.44

Sodium Thioglycolate

40000047-3 500 g
EUR 319.56

N-Butyl thioglycolate

B30910 100G
EUR 104.8
Description: CAS N° 10047-28-6

Sodium thioglycolate, 98%

GC0633 25g
EUR 304.24

Sodium thioglycolate, 98%

GC0633-1 1
EUR 332.2

Sodium thioglycolate, 98%

GC0633-100 100
EUR 47.4

Sodium thioglycolate, 98%

GC0633-100G 100 g
EUR 93.6

Sodium thioglycolate, 98%

GC0633-1KG 1 kg
EUR 438

Sodium thioglycolate, 98%

GC0633-25 25
EUR 16.6

Sodium thioglycolate, 98%

GC0633-250 250
EUR 102.7

Sodium thioglycolate, 98%

GC0633-250G 250 g
EUR 160.8

Sodium thioglycolate, 98%

GC0633-25G 25 g
EUR 56.4

Sodium thioglycolate, 98%

GC0633-500 500
EUR 189.8

Sodium thioglycolate, 98%

GC0633-500G 500 g
EUR 265.2

Methyl thioglycolate 98%

M28950 25G
EUR 75.32
Description: CAS N° 2365-48-2

Sodium thioglycolate

MBS5759319-5mg 5mg
EUR 145

Sodium thioglycolate

MBS5759319-5x5mg 5x5mg
EUR 500

Sodium thioglycolate

T8530-10mg 10mg Ask for price
Description: Sodium thioglycolate

Sodium thioglycolate

T8530-1g 1g Ask for price
Description: Sodium thioglycolate

Sodium thioglycolate

T8530-1mg 1mg Ask for price
Description: Sodium thioglycolate

Sodium thioglycolate

T8530-50mg 50mg Ask for price
Description: Sodium thioglycolate

Sodium thioglycolate

T8530-5mg 5mg Ask for price
Description: Sodium thioglycolate

Sodium thioglycolate

S0265 25g
EUR 68.29

Sodium Thioglycolate

1191450 100 100 g Ask for price

Sodium Thioglycolate

1191450 500 500 g Ask for price

Sodium Thioglycolate

S5190
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  • 5 g
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  • 2.5 Kg

Tridecyl thioglycolate

T21175 25G
EUR 90
Description: CAS N° 50727-78-1

Potassium thioglycolate 98%

P24000 5G
EUR 85.61
Description: CAS N° 34452-51-2

Thioglycolic acid sodium salt

T11098 25G
EUR 123.24
Description: CAS N° 367-51-1

Human Tubal Fluid (HTF) Mouse Embryo Medium

TBS8072-50ML 50mL
EUR 39

3-Fluorophenylethylammonium Thioglycolate

F325610 100mg
EUR 800

3-Fluorophenylethylammonium Thioglycolate

TRC-F325610-100MG 100 mg Ask for price

PolyFreeze (Tissue Freezing Medium) - Clear

19636-1 1case
EUR 248.4

PolyFreeze (Tissue Freezing Medium) - Clear

19636-4 4oz
EUR 35.64

Ethanolamine thioglycolate

E02860 500G
EUR 275.6
Description: CAS N° 126-97-6

Sodium Thioglycolate extrapure, 99%

78786 100 Gms
EUR 7.28
Description: Part B

Methylenebis(thioglycolic)acid

M16840 100G
EUR 172.72
Description: CAS N° 2068-24-8

Stearyl thioglycolate_x000D__x000D_

S09140 25G
EUR 165.36
Description: CAS N° 10220-46-9

n-Octylammonium Thioglycolate

O547830 100mg
EUR 800

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