October 30, 2020
bioinfogenome

Conditional reprogramming: subsequent technology cellculture

Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian CellCultures

 

Organic organisms are continuously uncovered to an immense repertoire of molecules that cowl environmental or food-derived molecules and medicines, triggering a steady movement of stimuli-dependent diversifications.

  • The variety of those chemical substances in addition to their concentrations contribute to the multiplicity of induced results, together with activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, because the foremost phenotype and manifestation of life, has confirmed to be immensely delicate and extremely adaptive to chemical stimuli.
  • Subsequently, learning the impact of endo- or xenobiotics over mobile metabolism delivers priceless information to apprehend potential mobile exercise of particular person molecules and consider their acute or power advantages and toxicity.
  • The event of contemporary metabolomics applied sciences akin to mass spectrometry or nuclear magnetic resonance spectroscopy now gives unprecedented options for the speedy and environment friendly willpower of metabolic profiles of cells and extra complicated organic methods. Mixed with the supply of well-established cell tradition strategies, these analytical strategies seem completely suited to find out the organic exercise and estimate the constructive and adverse results of chemical substances in quite a lot of cell sorts and fashions, even at hardly detectable concentrations.
  • Metabolic phenotypes will be estimated from learning intracellular metabolites at homeostasis in vivo, whereas in vitro cell cultures present extra entry to metabolites exchanged with progress media.
  • This text discusses analytical options out there for metabolic phenotyping of cell tradition metabolism in addition to the overall metabolomics workflow appropriate for testing the organic exercise of molecular compounds.
  • We emphasize how metabolic profiling of cell supernatants and intracellular extracts can ship priceless and complementary insights for evaluating the consequences of xenobiotics on mobile metabolism. We word that the ideas and strategies mentioned primarily for xenobiotics publicity are extensively relevant to drug testing on the whole, together with endobiotics that cowl energetic metabolites, vitamins, peptides and proteins, cytokines, hormones, nutritional vitamins, and so on.
bioinfogenome
bioinfogenome
FluoreGuard Mounting Medium
FMM500 500 ml
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FluoreGuard Mounting Medium
FMM999 1000 ml
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SuperMOUNT? Mounting Medium
1211-20
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EverBrite Mounting Medium
23001 10mL
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Description: Minimum order quantity: 1 unit of 10mL
Fluorguard Antifade Mounting Medium
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Anti-Fade Mounting Medium
abx090692-10ml 10 ml
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EverBrite Hardset Mounting Medium
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NB300 60 ml
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NB300-30 30 ml
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Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 125ml
NB300A 125 ml
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FluoreGuard Mounting Medium (Hard Set)
FMH030 30 ml
EUR 96
FluoreGuard Mounting Medium (Hard Set)
FMH060 60 ml
EUR 116
FluoreGuard Mounting Medium (Hard Set)
FMH500 500 ml
EUR 327
FluoreGuard Mounting Medium (Hard Set)
FMH999 1000 ml
EUR 530
EverBrite Mounting Medium with DAPI
23002 10mL
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abx090690-10ml 10 ml
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abx090691-10ml 10 ml
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23004 10mL
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20004 50 mL
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FluorLast? Mounting Media
1213-20
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GMM003 3 ml
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Buffered Glycerol Mounting Media
GMM030 30 ml
EUR 72
Buffered Glycerol Mounting Media
GMM500 500 ml
EUR 183
Buffered Glycerol Mounting Media
GMM999 1000 ml
EUR 260
Permanent Mounting Media (Aqueous)
PMT030 30 ml
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Permanent Mounting Media (Aqueous)
PMT999 1000 ml
EUR 759
ReadiUse™ microscope mounting solution
20009 50 mL
EUR 115
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PO501 each
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Description: Best quality products
Advanced Medium
C0003-04 RT 500 mL Bottle
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Medium 199
C0012-01 RT 500 mL Bottle
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COLIFORM MEDIUM
C03-127-10kg 10 kg
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COLIFORM MEDIUM
C03-127-2kg 2kg
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COLIFORM MEDIUM
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EC MEDIUM
E05-100-10kg 10 kg
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EC MEDIUM
E05-100-2Kg 2 Kg
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EC MEDIUM
E05-100-500g 500 g
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HLP MEDIUM
H08-107-10kg 10 kg
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HLP MEDIUM
H08-107-2Kg 2 Kg
EUR 534
HLP MEDIUM
H08-107-500g 500 g
EUR 182
VALI Medium
450 500 ml
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BEGM Medium
451 500 ml
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A Medium
DJ1018 100g
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  • Product category: Culture Media/Medium
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D04-117-10kg 10 kg
EUR 1579
DC MEDIUM
D04-117-2kg 2kg
EUR 382
DC MEDIUM
D04-117-500g 500 g
EUR 140
Heller's Medium
CP014-010 10X1L
EUR 99
Heller's Medium
CP014-500 50L
EUR 126
Hoagland's Medium
CP015-010 10X1L
EUR 99
Hoagland's Medium
CP015-500 50L
EUR 126
NeuroProgenitor Medium
NM42400 125 ml
EUR 304
SIM MEDIUM
S19-110-10kg 10 kg
EUR 1021
SIM MEDIUM
S19-110-2kg 2kg
EUR 261
SIM MEDIUM
S19-110-500g 500 g
EUR 107
SOB MEDIUM
S19-124-10kg 10 kg
EUR 965
SOB MEDIUM
S19-124-2kg 2kg
EUR 249
SOB MEDIUM
S19-124-500g 500 g
EUR 104
M9 Medium
SD7024 250g
EUR 71.75
  • Product category: Culture Media/Medium
M9CA Medium
SD7025 250g
EUR 71.75
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YM Medium
SD7031 250g
EUR 70.45
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TYGPN Medium
SD7032 250g
EUR 70.01
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M63 Medium
SD7033 250g
EUR 71.75
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Probe Mount, Permanent Mounting media for permanent mounting and coverslipping of Special stainsd & Immunofluorescence and In-Situ Stains 60ml
NB316 60 ml
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Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001 1 liter Ask for price
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C0002-20mL RT vial
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C03-131-2Kg 2 Kg
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E05-108-10kg 10 kg
EUR 858
EC MEDIUM, MODIFIED
E05-108-2Kg 2 Kg
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EC MEDIUM, MODIFIED
E05-108-500g 500 g
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Hepatocyte Maintenance Medium
HM42600 100 ml
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Neuron Growth Medium
HNM001 500 ml
EUR 331
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GE4750-100G 100 g
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GE4750-500G 500 g
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F06-101-10kg 10 kg
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F06-101-2kg 2kg
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F06-101-500g 500 g
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RPMI 1640 Medium
abx098879-500ml 500 ml
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A01-116-10kg 10 kg
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A-1 MEDIUM
A01-116-2Kg 2 Kg
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A-1 MEDIUM
A01-116-500g 500 g
EUR 108
Recovery Medium - 6x12mL
1711 4/PK
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H Medium Broth
DJ1019 100g
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MCDB 131 Medium
CM034-050 500ml
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MCDB 131 Medium
CM034-300 6x500ml
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MCDB 131 Medium
CM034-310 10x500ml
EUR 219
MCDB 131 Medium
CM034-320 20x500ml
EUR 340
MCDB 131 Medium
CM034-350 50x500ml
EUR 585
Williams' Medium E
CM063-050 500ml
EUR 91
Williams' Medium E
CM063-300 6x500ml
EUR 266

Conditional reprogramming: subsequent technology cellculture

Lengthy-term major tradition of mammalian cells has been at all times tough attributable to unavoidable senescence. Standard strategies for producing immortalized cell strains normally require manipulation of genome which ends up in change of vital organic and genetic traits. Not too long ago, conditional reprogramming (CR) emerges as a novel subsequent technology instrument for long-term tradition of major epithelium cells derived from nearly all origins with out alteration of genetic background of major cells.

CR co-cultures major cells with inactivated mouse 3T3-J2 fibroblasts within the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling major cells to accumulate stem-like traits whereas retain their potential to completely differentiate. With only some years’ growth, CR exhibits broad prospects in purposes in diversified areas together with illness modeling, regenerative drugs, drug analysis, drug discovery in addition to precision drugs. This evaluate is thus to comprehensively summarize and assess present progress in understanding mechanism of CR and its broad purposes, highlighting the worth of CR in each primary and translational researches and discussing the challenges confronted with CR.

Results of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a mannequin extremely glycosylated therapeutic protein, in a transgenic rice cell suspension tradition handled with kifunensine, a powerful α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

A media trade was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and including recent sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to provide rice recombinant human BChE (rrBChE). Utilizing a 1.25X-concentrated sugar-free medium along with an 80% lowered working quantity through the media trade led to a complete energetic rrBChE manufacturing degree of 79 ± 2 µg (g FW)-1 or 7.5 ± 0.Four mg L-1 within the presence of kifunensine, which was 1.5-times greater than our earlier bioreactor runs utilizing regular sugar-free (NB-S) media with no kifunensine remedy.

Importantly, the quantity of secreted energetic rrBChE in tradition medium was enhanced within the presence of kifunensine, comprising 44% of the entire energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine remedy, which was attributed to completely different N-glycoforms. N-Glycosylation evaluation confirmed considerably elevated oligomannose glycans (Man5/6/7/8) in rrBChE handled with kifunensine in comparison with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the main purpose for incomplete inhibition of α-mannosidase I on this bioreactor examine.

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-500g 500 g
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SODIUM THIOGLYCOLLATE

S19-136-10kg 10 kg Ask for price

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A01-103-2Kg 2 Kg Ask for price

ALT. THIOGLYCOLLATE MED.

A01-103-500g 500 g Ask for price

Bouin's Fluid

BNF125 125 ml
EUR 63

Bouin's Fluid

BNF3800 1 Gal.
EUR 178

Bouin's Fluid

BNF500 500 ml
EUR 80

Bouin's Fluid

BNF999 1000 ml
EUR 98

Rat Neuromedin U control/blocking peptide # 2

NMU51-P 100 ug
EUR 164

Human Neuromedin U control/blocking peptide # 3

NMU61-P 100 ug
EUR 164

Mouse Neuromedin U control/blocking peptide # 4

NMU71-P 100 ug
EUR 164

P-Selectin (PF377), RNA Aptamer, unlabeled

AR-298-U Custom Ask for price

Michel's Transport Fluid

MTF-10000 10 L
EUR 762

Michel's Transport Fluid

MTF-20000 20 L
EUR 1340

Michel's Transport Fluid

MTF500 500 ml
EUR 98

Michel's Transport Fluid

MTF999 1000 ml
EUR 127

Ansamitocin P-3

ADC-P-003 unit Ask for price

Neuromedin U (R/M/H) control/blocking peptides

NMU41-P 100 ug
EUR 164

SOF Synthetic Oviduct Fluid.

IVL05-100ML 100 ml
EUR 78
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  • Product family: Bovine IVF Media
Description: Synthetic Oviduct Fluid.

SOF Synthetic Oviduct Fluid.

IVL05-6X100ML 6 x 100 ml
EUR 151
  • Product line: Animal Cell Culture Media
  • Product family: Bovine IVF Media
Description: Synthetic Oviduct Fluid.

HE4, Human ascites fluid

P1438-100
EUR 620

HE4, Human ascites fluid

P1438-500
EUR 2839

AXYPREP MAG PLASMID KIT- MEDIUM - 384 PREPS

MAG-P-M 1/pk
EUR 664
Description: Bioscience Mag Beads; Magnetic Plasmid

Transparent, 96-Well Plates, pureGrade Sterile, Clear U-Bottom 50 Plates/Unit

91-420U 50 plates
EUR 230
Description:
  • Non-treated medium binding surface
  • 100% high quality polystyrene
  • Individually-wrapped plates w/ lids
  • Sterilized via beta-radiation
  • Available in clear, white or black
  • Free from endotoxins, DNase, DNA, RNase; noncytotoxic

96-Well, pureGrade, Clear Transparent, U-Bottom, 5 Plates/Sleeve, 100/Unit

33-572X 100 plates
EUR 240
Description:
  • Clear for colorimetric assays
  • Non-treated "medium binding" surface
  • 100% high quality polystyrene
  • 330µl well volume
  • Non-sterile
  • 1 lid included in each stack (20 total lids)
  • 20 stacks of 5 plates, pack of 100

YLKKIKNSL, P. falciparum circumsporozoite (CSP 334-342) polypeptide

CSPF15-P 1 mg
EUR 286

Mouse p/CIP/p300 Control/blocking peptide # 1

CIP11-P 100 ug
EUR 164

Mouse p/CIP/p300 Control/blocking peptide # 2

CIP12-P 100 ug
EUR 164

Hepatitis B Virus (HBV) Polymerase (P protein) (A9), RNA Aptamer, unlabeled

AR-233-U Custom Ask for price

Calci-Clear

NAT1312 EACH
EUR 78

Calci-Clear

NAT1314 EACH
EUR 128

Calci-Clear

NAT1316 EACH
EUR 386

Histo-Clear

NAT1330 EACH
EUR 154

Histo-Clear

NAT1332 EACH
EUR 583

LEYYLREKAKMAGTLIIPES, P. vivax PvMSP-1 peptide 19 (378-397)

MSPV11-P 1 mg
EUR 164

SKDQIKKLTSLKNKLERRQN, P. vivax PvMSP-1 peptide 53 (1058-1077)

MSPV12-P 1 mg
EUR 164

NFVGKFLELQIPGHTDLLHL, P. vivax PvMSP-1 peptide 4 (78-97)

MSPV13-P 1 mg
EUR 164

FNQLMHVINFHYDLLRANVH, P. vivax PvMSP-1 peptide 6 (118-137)

MSPV14-P 1 mg
EUR 164

LDMLKKVVLGLWKPLDNIKD, P. vivax PvMSP-1 peptide 8 (158-177)

MSPV15-P 1 mg
EUR 164

Human Substance P Peptide (amide salt)

SP15-P-1 1 mg
EUR 164

Human Substance P Peptide (amide salt)

SP15-P-25 25 mg
EUR 529

Human Substance P Peptide (amide salt)

SP15-P-5 5 mg
EUR 286

CA-125, Human Ascites Fluid

P1446-10
EUR 294

MICROPLATE, 96 WELL, POLYSTYRENE, CLEAR, FLAT BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, BULK

9017 25/pk
EUR 179
Description: Assay; General Assay - 96 Well

MICROPLATE, 96 WELL, POLYSTYRENE, CLEAR, ROUND BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, BULK

3797 25/pk
EUR 179
Description: Assay; EIA/RIA - 96 Well

KIYNRNIVNRLLGD, P-yoelii circumsporozoite protein (57?70), PyCSP (57?70) peptide

CSPY11-P 1 mg
EUR 286

SYVPSAEQI, P-yoelii circumsporozoite protein (280?288), PyCSP (280?288) peptide

CSPY12-P 1 mg
EUR 286

DELFNELLNSVDVNGENILEESQ, P. falciparum Liver-Stage Antigen 3-NRI (LSA3-NRI) peptide

LSPF32-P 1 mg
EUR 286

U-44069

C5324-1 1 mg
EUR 224
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U-44069

C5324-10 10 mg
EUR 1442
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U-44069

C5324-5 5 mg
EUR 833
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U-93631

B1851-25
EUR 544

U-93631

B1851-5
EUR 175

U-73122

B1887-25
EUR 544

U-73122

B1887-5
EUR 175

U-73343

B1888-5
EUR 175

U-73122

B3422-10 10 mg
EUR 161
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-25 25 mg
EUR 258
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-5 5 mg
EUR 123
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-5.1 10 mM (in 1mL DMSO)
EUR 166
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U 18666A

B6812-10 10 mg
EUR 150
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 18666A

B6812-25 25 mg
EUR 287
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 18666A

B6812-50 50 mg
EUR 473
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 46619

B6890-1 1 mg
EUR 147
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

U 46619

B6890-10 10 mg
EUR 830
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

U 46619

B6890-5 5 mg
EUR 487
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

U 90042

B7164-10 10 mg
EUR 350

U 90042

B7164-50 50 mg
EUR 1266

U 89843A

B7165-10 10 mg
EUR 292

U 89843A

B7165-50 50 mg
EUR 1054

U 93631

B7170-10 10 mg
EUR 248

U 93631

B7170-100 100 mg
EUR 1407

U 93631

B7170-5 5 mg
EUR 195

U 93631

B7170-5.1 10 mM (in 1mL DMSO)
EUR 209

U 93631

B7170-50 50 mg
EUR 864

U 73343

B9013-10 10 mg
EUR 224

U 73343

B9013-25 25 mg
EUR 441

U 73343

B9013-5 5 mg
EUR 154

U 0126

E1B1901 1mg
EUR 161

U-73343

HY-108630 5mg
EUR 234

U-101017

HY-19250 1mg
EUR 1125

U-73122

HY-13419 10mM/1mL
EUR 147

U-104

HY-13513 10mM/1mL
EUR 113

U-74389G

20-abx076631
  • EUR 314.00
  • EUR 857.00
  • 100 mg
  • 500 mg
  • Shipped within 5-12 working days.

U-0126

20-abx076796
  • EUR 1121.00
  • EUR 384.00
  • 25 mg
  • 5 mg
  • Shipped within 5-12 working days.

U-18666A

9469-100 Ask for price

U-18666A

9469-25
EUR 457

U-18666A

9469-5
EUR 153

U-104

9631-25
EUR 414

U-104

9631-5
EUR 142

U-104

A4358-10 10 mg
EUR 108
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-25 25 mg
EUR 171
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-5.1 10 mM (in 1mL DMSO)
EUR 108
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-50 50 mg
EUR 270
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

Kazinol U

TBW00590 unit Ask for price

Gomisin U

TBW01615 5mg Ask for price

Ixerin U

TBZ1502 unit Ask for price

MICROPLATE, 96 WELL, POLYSTYRENE, CLEAR, FLAT BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, INDIVIDUALLY PACKAGED

3591 1/pk
EUR 130
Description: Assay; EIA/RIA - 96 Well

EZ-10 RNA Column and collection tube (clear tube, clear ring, clear collection)

SD5008 100, 100UNIT
EUR 75.88
  • Product category: Labware/Columns/Spin Columns

VTHESYQELVKKLEALEDAV, MSP-1 P1, peptide of P. falciparum Merozoite Surface Protein 1

MSPF11-P 1 mg
EUR 225

GYRKPLDNIKDNVGKMEDYIKK, MSP-1 P2, peptide of P. falciparum Merozoite Surface Protein 1

MSPF12-P 1 mg
EUR 225

KLNSLNNPHNVLQNFSVFFNK, MSP-1 P3, peptide of P. falciparum Merozoite Surface Protein 1

MSPF131-P 1 mg
EUR 225

384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR

PCR-384-RGD-C 20/pk
EUR 459
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Exosome Isolation kit (forHuman Body Fluid)

P121 NULL
EUR 0

Exosome Isolation kit (forHuman Body Fluid)

P121L NULL
EUR 0

Exosome Isolation kit (forHuman Body Fluid)

P121S NULL
EUR 0

Cancer Antigen 125 Antigen (Ascites Fluid)

VAng-Cr3870-10kU 10 kU
EUR 944
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE.

Calci-Clear Rapid

NAT1318 EACH
EUR 75

Calci-Clear Rapid

NAT1320 EACH
EUR 128

Calci-Clear Rapid

NAT1322 EACH
EUR 386

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