Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian CellCultures

 

Organic organisms are continuously uncovered to an immense repertoire of molecules that cowl environmental or food-derived molecules and medicines, triggering a steady movement of stimuli-dependent diversifications.

• The variety of those chemical substances in addition to their concentrations contribute to the multiplicity of induced results, together with activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, because the foremost phenotype and manifestation of life, has confirmed to be immensely delicate and extremely adaptive to chemical stimuli.

• Subsequently, learning the impact of endo- or xenobiotics over mobile metabolism delivers priceless information to apprehend potential mobile exercise of particular person molecules and consider their acute or power advantages and toxicity.

• The event of contemporary metabolomics applied sciences akin to mass spectrometry or nuclear magnetic resonance spectroscopy now gives unprecedented options for the speedy and environment friendly willpower of metabolic profiles of cells and extra complicated organic methods. Mixed with the supply of well-established cell tradition strategies, these analytical strategies seem completely suited to find out the organic exercise and estimate the constructive and adverse results of chemical substances in quite a lot of cell sorts and fashions, even at hardly detectable concentrations.

• Metabolic phenotypes will be estimated from learning intracellular metabolites at homeostasis in vivo, whereas in vitro cell cultures present extra entry to metabolites exchanged with progress media.

• This text discusses analytical options out there for metabolic phenotyping of cell tradition metabolism in addition to the overall metabolomics workflow appropriate for testing the organic exercise of molecular compounds.

• We emphasize how metabolic profiling of cell supernatants and intracellular extracts can ship priceless and complementary insights for evaluating the consequences of xenobiotics on mobile metabolism. We word that the ideas and strategies mentioned primarily for xenobiotics publicity are extensively relevant to drug testing on the whole, together with endobiotics that cowl energetic metabolites, vitamins, peptides and proteins, cytokines, hormones, nutritional vitamins, and so on.

FluoreGuard Mounting Medium
FMM060	ScyTek Laboratories	60 ml	EUR 139.2

FluoreGuard Mounting Medium
FMM500	ScyTek Laboratories	500 ml	EUR 392.4

FluoreGuard Mounting Medium
FMM999	ScyTek Laboratories	1000 ml	EUR 636

Anti-Fade Mounting Medium
abx090692-10ml	Abbexa	10 ml	EUR 243.6

EverBrite Hardset Mounting Medium
23003	Biotium	10mL	EUR 234
Description: Minimum order quantity: 1 unit of 10mL

Fluorguard Antifade Mounting Medium
F001	Insitus Biotechnologies	1 ml	EUR 103.2

FluoroQuestâ„¢ Antifade Mounting Medium
20007-5mL	AAT Bioquest	5 mL	EUR 109
Description: FluoroQuestâ„¢ Antifade Mounting Medium is a specialized and stable formula that effectively preserves fluorescence by preventing rapid photobleaching of fluorescent proteins and dyes.

FluoreGuard Mounting Medium (Hard Set)
FMH030	ScyTek Laboratories	30 ml	EUR 115.2

FluoreGuard Mounting Medium (Hard Set)
FMH060	ScyTek Laboratories	60 ml	EUR 139.2

FluoreGuard Mounting Medium (Hard Set)
FMH500	ScyTek Laboratories	500 ml	EUR 392.4

FluoreGuard Mounting Medium (Hard Set)
FMH999	ScyTek Laboratories	1000 ml	EUR 636

FluoroQuestâ„¢ Mounting Medium with DAPI
20004-50mL	AAT Bioquest	50 mL	EUR 109
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Anti-Fade Mounting Medium (With PI)
abx090691-10ml	Abbexa	10 ml	EUR 243.6

Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml	Abbexa	10 ml	EUR 243.6

FluoroQuestâ„¢ PLUS Antifade Mounting Medium
20008-5mL	AAT Bioquest	5 mL	EUR 129
Description: FluoroQuestâ„¢ PLUS Antifade Mounting Medium, the latest breakthrough in non-setting media technology, provides unparalleled protection against fading throughout the visible spectrum, including far-red wavelengths.

FluoroQuest™ Mounting Medium with DAPI
20004	AAT Bioquest	50 mL	EUR 109

EverBrite Hardset Mounting Medium with DAPI
23004	Biotium	10mL	EUR 255.6
Description: Minimum order quantity: 1 unit of 10mL

FluoroQuestâ„¢ Anti-fading Mounting Medium with DAPI
20005-20mL	AAT Bioquest	20 mL	EUR 211
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

FluoroQuest™ Anti-fading Mounting Medium with DAPI
20005	AAT Bioquest	20 mL	EUR 211

Elix½ Advantage 3
ZRXV003T0	Scientific Laboratory Supplies	EACH	EUR 9260.62

Elix½ Advantage 5
ZRXV005T0	Scientific Laboratory Supplies	EACH	EUR 10995.76

Elix½ Advantage 10
ZRXV010T0	Scientific Laboratory Supplies	EACH	EUR 12220.42

Elix½ Advantage 15
ZRXV015T0	Scientific Laboratory Supplies	EACH	EUR 13232.26

Milli-Q½ Advantage A10
Z00Q0V0T0	Scientific Laboratory Supplies	EACH	EUR 11562.39

BD 2 ml advantage pipet
356507	Scientific Laboratory Supplies	PK1000	EUR 242.4

ELIX ADVANTAGE UPGRADE KIT
ZRXSUP0D1	Scientific Laboratory Supplies	EACH	EUR 2249.55

BD 25 ml advantage pipette
356535	Scientific Laboratory Supplies	PK200	EUR 168

BD 1 ml 1100 advantage pipet
356521	Scientific Laboratory Supplies	PK1000	EUR 213.6

Mounting Media
99-MED	Fitzgerald	3 ml	EUR 253.2
Description: Mounting media for use in microscopy preparations

MSC Advantage 1.2 230V 50Hz EN Certified NF listed
CAB2662	Scientific Laboratory Supplies	EACH	EUR 12540

MSC Advantage 1.8 230V 50Hz EN Certified NF listed
CAB2664	Scientific Laboratory Supplies	EACH	EUR 15859.2

BD Advantage 5ml Serological Pipette Plugged
356543	Scientific Laboratory Supplies	PK200	EUR 82.8

BD Advantage 25ml Serological Pipette Plugged
356525	Scientific Laboratory Supplies	PK200	EUR 170.4

BD Advantage 50ml Serological Pipette Plugged
356550	Scientific Laboratory Supplies	PK100	EUR 182.4

BD Advantage 10ml Serological Pipette Plugged
356551	Scientific Laboratory Supplies	PK200	EUR 81.6

FluorLast? Mounting Media
1213-20	Biovision	each	EUR 170.4

Permanent Mounting Media (Aqueous)
PMT030	ScyTek Laboratories	30 ml	EUR 130.8

Permanent Mounting Media (Aqueous)
PMT999	ScyTek Laboratories	1000 ml	EUR 910.8

Buffered Glycerol Mounting Media
GMM003	ScyTek Laboratories	3 ml	EUR 73.2

Buffered Glycerol Mounting Media
GMM030	ScyTek Laboratories	30 ml	EUR 86.4

Buffered Glycerol Mounting Media
GMM500	ScyTek Laboratories	500 ml	EUR 219.6

Buffered Glycerol Mounting Media
GMM999	ScyTek Laboratories	1000 ml	EUR 312

Probe Mount, Permanent Mounting media for permanen
NB316	Innovex	60 ml	EUR 385
Description: Probe Mount, Permanent Mounting media for permanent mounting and coverslipping of Special stainsd & Immunofluorescence and In-Situ Stains FROM WATER, 60 ml

Probe Mount, Permanent Mounting media for permanen
NB316-30	Innovex	30 ml	EUR 275
Description: Probe Mount, Permanent Mounting media for permanent mounting and coverslipping of Special stainsd & Immunofluorescence and In-Situ Stains FROM WATER, 30 ml

PolyMount Mounting Media MaxTag Histo (OKRA00045)
OKRA00045	Aviva Systems Biology	15 ml	EUR 151.2
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

H&E Mount, Permanent Mountindg media for mounting
NB315	Innovex	125 ml	EUR 375
Description: H&E Mount, Permanent Mountindg media for mounting H&E stains from water, 125 ml (Large volume)

H&E Mount, Permanent Mountindg media for mounting
NB315-40	Innovex	40 ml	EUR 195
Description: H&E Mount, Permanent Mountindg media for mounting H&E stains from water, 40 ml (Small volume)

Wall Mounting Kit
WAT2452	Scientific Laboratory Supplies	EACH	EUR 160.59

Wall Mounting Kit
WAT2456	Scientific Laboratory Supplies	EACH	EUR 297.68

WALL MOUNTING BRACKET
WMBSMT002	Scientific Laboratory Supplies	EACH	EUR 211.51

MILLIPAK MOUNTING KITT
MPKMNTKIT	Scientific Laboratory Supplies	EACH	EUR 251.98

Elga Wall Mounting Kit
WAT4044	Scientific Laboratory Supplies	EACH	EUR 383.85

Elga Wall Mounting Kit
WAT4046	Scientific Laboratory Supplies	EACH	EUR 151.45

WALL MOUNTING BRACKET (1)
SYSTFIX01	Scientific Laboratory Supplies	EACH	EUR 382.54

Wall Mounting Kit (TANK30)
WAT2448	Scientific Laboratory Supplies	EACH	EUR 150.14

Wall Mounting Kit (TANK60)
WAT2450	Scientific Laboratory Supplies	EACH	EUR 156.67

OPTICAP WALL MOUNTING BRACKET
M0PMNTKIT	Scientific Laboratory Supplies	EACH	EUR 199.76

WALL MOUNTING BRACKET (1/P)
TANKFIX01	Scientific Laboratory Supplies	EACH	EUR 238.92

Elga Flex Wall Mounting Kit
WAT4170	Scientific Laboratory Supplies	EACH	EUR 466.1

WALL MOUNTING BRACKET TANKPE100
TANKFIXS1	Scientific Laboratory Supplies	EACH	EUR 782.05

WALL MOUNTING BRACKET W VALVE
WMBSMT001	Scientific Laboratory Supplies	EACH	EUR 278.09

Mounting Bracket For Base MIC2510
MIC2510	Scientific Laboratory Supplies	EACH	EUR 105.6

Advanced Medium
C0003-04	Addexbio	RT 500 mL Bottle	EUR 123.6

ReadiUseâ„¢ microscope mounting solution
20009-50mL	AAT Bioquest	50 mL	EUR 86
Description: The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

Holder for water tank for mounting
CHA4068	Scientific Laboratory Supplies	EACH	EUR 147.6

KL 300 LED Column Mounting Bracket
MIC2528	Scientific Laboratory Supplies	EACH	EUR 80.4

WALL MOUNTING BRACKET WASHER PUMP KIT
WMBWASH1	Scientific Laboratory Supplies	EACH	EUR 186.7

ReadiUse™ microscope mounting solution
20009	AAT Bioquest	50 mL	EUR 86

Fine natural hair brush for mounting sections
PO501	FD Neurotechnologies	each	EUR 80.4

MicroRT room temperature mounting kit 20 MicroRT tubes, razor blade and grease tube
RT-T1	MiTeGen	1 KIT	EUR 64
Description: MicroRT room temperature mounting kit20 MicroRT tubes, razor blade and grease tube

Magnetic platform, includes 4 metal mounting strips, accessory for Piggy? Tube Rotator
ACT-1040-6	ACTGene	each	EUR 356.4

Medium 199
C0012-01	Addexbio	RT 500 mL Bottle	EUR 126

MicroMount Medium Aperture Assortment - 5 each of 75, 100, 150 and 200 micron aperture mounts; mounted on 18 mm rods
M2-L18SP-A2	MiTeGen	20 MOUNTS	EUR 112
Description: MicroMount Medium Aperture Assortment - 5 each of 75, 100, 150 and 200 micron aperture mounts; mounted on 18 mm rods

DC MEDIUM
D04-117-10kg	Alphabiosciences	10 kg	EUR 1894.8

DC MEDIUM
D04-117-2kg	Alphabiosciences	2kg	EUR 458.4

DC MEDIUM
D04-117-500g	Alphabiosciences	500 g	EUR 168

A Medium
DJ1018	Bio Basic	100g	EUR 101.76

EC MEDIUM
E05-100-10kg	Alphabiosciences	10 kg	EUR 976.8

EC MEDIUM
E05-100-2Kg	Alphabiosciences	2 Kg	EUR 259.2

EC MEDIUM
E05-100-500g	Alphabiosciences	500 g	EUR 114

OF Medium
MED1664	Scientific Laboratory Supplies	500G	EUR 79.2

M9 Medium
SD7024	Bio Basic	250g	EUR 86.1

YM Medium
SD7031	Bio Basic	250g	EUR 84.54

HLP MEDIUM
H08-107-10kg	Alphabiosciences	10 kg	EUR 2733.6

HLP MEDIUM
H08-107-2Kg	Alphabiosciences	2 Kg	EUR 640.8

HLP MEDIUM
H08-107-500g	Alphabiosciences	500 g	EUR 218.4

M63 Medium
SD7033	Bio Basic	250g	EUR 86.1

SIM MEDIUM
S19-110-10kg	Alphabiosciences	10 kg	EUR 1225.2

SIM MEDIUM
S19-110-2kg	Alphabiosciences	2kg	EUR 313.2

SIM MEDIUM
S19-110-500g	Alphabiosciences	500 g	EUR 128.4

SOB MEDIUM
S19-124-10kg	Alphabiosciences	10 kg	EUR 1158

SOB MEDIUM
S19-124-2kg	Alphabiosciences	2kg	EUR 298.8

SOB MEDIUM
S19-124-500g	Alphabiosciences	500 g	EUR 124.8

Conditional reprogramming: subsequent technology cellculture

Lengthy-term major tradition of mammalian cells has been at all times tough attributable to unavoidable senescence. Standard strategies for producing immortalized cell strains normally require manipulation of genome which ends up in change of vital organic and genetic traits. Not too long ago, conditional reprogramming (CR) emerges as a novel subsequent technology instrument for long-term tradition of major epithelium cells derived from nearly all origins with out alteration of genetic background of major cells.

CR co-cultures major cells with inactivated mouse 3T3-J2 fibroblasts within the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling major cells to accumulate stem-like traits whereas retain their potential to completely differentiate. With only some years’ growth, CR exhibits broad prospects in purposes in diversified areas together with illness modeling, regenerative drugs, drug analysis, drug discovery in addition to precision drugs. This evaluate is thus to comprehensively summarize and assess present progress in understanding mechanism of CR and its broad purposes, highlighting the worth of CR in each primary and translational researches and discussing the challenges confronted with CR.

Results of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a mannequin extremely glycosylated therapeutic protein, in a transgenic rice cell suspension tradition handled with kifunensine, a powerful α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

A media trade was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and including recent sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to provide rice recombinant human BChE (rrBChE). Utilizing a 1.25X-concentrated sugar-free medium along with an 80% lowered working quantity through the media trade led to a complete energetic rrBChE manufacturing degree of 79 ± 2 µg (g FW)^-1 or 7.5 ± 0.Four mg L^-1 within the presence of kifunensine, which was 1.5-times greater than our earlier bioreactor runs utilizing regular sugar-free (NB-S) media with no kifunensine remedy.

Importantly, the quantity of secreted energetic rrBChE in tradition medium was enhanced within the presence of kifunensine, comprising 44% of the entire energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine remedy, which was attributed to completely different N-glycoforms. N-Glycosylation evaluation confirmed considerably elevated oligomannose glycans (Man5/6/7/8) in rrBChE handled with kifunensine in comparison with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the main purpose for incomplete inhibition of α-mannosidase I on this bioreactor examine.

SODIUM THIOGLYCOLLATE
S19-136-10kg	Alphabiosciences	10 kg	Ask for price

SODIUM THIOGLYCOLLATE
S19-136-2Kg	Alphabiosciences	2 Kg	Ask for price

SODIUM THIOGLYCOLLATE
S19-136-500g	Alphabiosciences	500 g	Ask for price

Fluid Thiogly Medium (USP)
MED1472	Scientific Laboratory Supplies	EACH	EUR 80.4

Fluid Thio Medium 500gm
225650	Scientific Laboratory Supplies	EACH	EUR 139.2

Thioglycolic acid
GK6903-1L	Glentham Life Sciences	1 l	EUR 175.2

Thioglycolic acid
GK6903-2500ML	Glentham Life Sciences	2500 ml	EUR 313.2

Thioglycolic acid
GK6903-250ML	Glentham Life Sciences	250 ml	EUR 74.4

Thioglycolic acid
GK6903-500ML	Glentham Life Sciences	500 ml	EUR 108

Thioglycolic acid
GK6903-1	Glentham Life Sciences	1	EUR 114.6

Thioglycolic acid
GK6903-250	Glentham Life Sciences	250	EUR 31.7

Thioglycolic acid
GK6903-2500	Glentham Life Sciences	2500	EUR 229.3

Thioglycolic acid
GK6903-500	Glentham Life Sciences	500	EUR 59.3

Sodium thioglycolate, 98%
GC0633-100G	Glentham Life Sciences	100 g	EUR 93.6

Sodium thioglycolate, 98%
GC0633-1KG	Glentham Life Sciences	1 kg	EUR 438

Sodium thioglycolate, 98%
GC0633-250G	Glentham Life Sciences	250 g	EUR 160.8

Sodium thioglycolate, 98%
GC0633-25G	Glentham Life Sciences	25 g	EUR 56.4

Sodium thioglycolate, 98%
GC0633-500G	Glentham Life Sciences	500 g	EUR 265.2

Sodium thioglycolate
S0265	Bio Basic	25g	EUR 68.29

Sodium thioglycolate, 98%
GC0633-1	Glentham Life Sciences	1	EUR 332.2

Sodium thioglycolate, 98%
GC0633-100	Glentham Life Sciences	100	EUR 47.4

Sodium thioglycolate, 98%
GC0633-25	Glentham Life Sciences	25	EUR 16.6

Sodium thioglycolate, 98%
GC0633-250	Glentham Life Sciences	250	EUR 102.7

Sodium thioglycolate, 98%
GC0633-500	Glentham Life Sciences	500	EUR 189.8

Bags Clear Medium Duty 725x975mm
BAG2202	Scientific Laboratory Supplies	PK200	EUR 50.27

Thioglycine
GW6823-1	Glentham Life Sciences	1	EUR 145.5

Thioglycine
GW6823-5	Glentham Life Sciences	5	EUR 464

1-Thioglycerol
GC2938-100	Glentham Life Sciences	100	EUR 78.2

1-Thioglycerol
GC2938-25	Glentham Life Sciences	25	EUR 30.7

3-D Life Thioglycerol
T10-3	Cellendes	180 µl	EUR 57.6

Thiogly Med Brewer 500
211716	Scientific Laboratory Supplies	EACH	EUR 216

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, MEDIUM BINDING, OEM, NO LID, NONSTERILE, BULK
92593	CORNING	25/pk	EUR 814.8
Description: Stripwells; OEM - Strips

Diss Media Diss (JP) 2nd Diss Fluid
DB15-10L	Scientific Laboratory Supplies	10L	EUR 187.2

Diss Media Diss (JP) 1st Diss Fluid
DB18-10	Scientific Laboratory Supplies	10L	EUR 249.6

Diss Media Diss (JP) 2nd Diss Fluid - 1L
DB15-121	Scientific Laboratory Supplies	PK12	EUR 294

Diss Media Diss (JP) 1st Diss Fluid - 1L
DB18-121	Scientific Laboratory Supplies	PK12	EUR 294

Diss Media Conc 2nd Dissolution Fluid (250mL to 10L)
DBC15-250	Scientific Laboratory Supplies	PK12	EUR 554.4

RESERVOIR AXYVOIR, 384 PRISM BOTTOM SINGLE WELL, FOR 384 HEADS FROM BECKMANN CALIPER AND OTHERS, POLYPROPYLENE, CLEAR, MEDIUM PROFILE, NONSTERILE, BULK
RES-SW384-FX	CORNING	25/pk	EUR 225.6
Description: Reservoirs; Reservoirs - Axygen

Medium 199
C0012-01	Addexbio	RT 500 mL Bottle	EUR 126

Diss Media Diss Sim Gastric Fluid (M) at pH 1.25
DB125-10	Scientific Laboratory Supplies	10L	EUR 249.6

96 Well MTP U Shaped Clear
PCR0226	Scientific Laboratory Supplies	PK100	EUR 199.2

Diss Media Conc 2nd Dissolution Fluid (1L to 25L)
DBC15-960	Scientific Laboratory Supplies	PK6	EUR 420

Bouin's Fluid
BNF125	ScyTek Laboratories	125 ml	EUR 75.6

Bouin's Fluid
BNF3800	ScyTek Laboratories	1 Gal.	EUR 213.6

Bouin's Fluid
BNF500	ScyTek Laboratories	500 ml	EUR 96

Bouin's Fluid
BNF999	ScyTek Laboratories	1000 ml	EUR 117.6

FluoreGuard Mounting Medium
FMM030	ScyTek Laboratories	30 ml	EUR 115.2

FluoreGuard Mounting Medium
FMM060	ScyTek Laboratories	60 ml	EUR 139.2

FluoreGuard Mounting Medium
FMM500	ScyTek Laboratories	500 ml	EUR 392.4

FluoreGuard Mounting Medium
FMM999	ScyTek Laboratories	1000 ml	EUR 636

Hayems Fluid
RRSP6609-D	Atom Scientific	500ml	EUR 9.71

Pereneys Fluid
PFLH	Scientific Laboratory Supplies	500ML	EUR 182.78

DC MEDIUM
D04-117-10kg	Alphabiosciences	10 kg	EUR 1894.8

DC MEDIUM
D04-117-2kg	Alphabiosciences	2kg	EUR 458.4

DC MEDIUM
D04-117-500g	Alphabiosciences	500 g	EUR 168

A Medium
DJ1018	Bio Basic	100g	EUR 101.76

EC MEDIUM
E05-100-10kg	Alphabiosciences	10 kg	EUR 976.8

EC MEDIUM
E05-100-2Kg	Alphabiosciences	2 Kg	EUR 259.2

EC MEDIUM
E05-100-500g	Alphabiosciences	500 g	EUR 114

OF Medium
MED1664	Scientific Laboratory Supplies	500G	EUR 79.2

M9 Medium
SD7024	Bio Basic	250g	EUR 86.1

YM Medium
SD7031	Bio Basic	250g	EUR 84.54

RESERVOIR AXYVOIR, 384 PRISM BOTTOM SINGLE WELL, FOR 384 HEADS FROM BECKMANN CALIPER AND OTHERS, POLYPROPYLENE, CLEAR, MEDIUM PROFILE, STERILE, INDIVIDUALLY PACKAGED
RES-SW384-FX-SI	CORNING	1/pk	EUR 262.8
Description: Reservoirs; Reservoirs - Axygen

HLP MEDIUM
H08-107-10kg	Alphabiosciences	10 kg	EUR 2733.6

HLP MEDIUM
H08-107-2Kg	Alphabiosciences	2 Kg	EUR 640.8

HLP MEDIUM
H08-107-500g	Alphabiosciences	500 g	EUR 218.4

M63 Medium
SD7033	Bio Basic	250g	EUR 86.1

SIM MEDIUM
S19-110-10kg	Alphabiosciences	10 kg	EUR 1225.2

SIM MEDIUM
S19-110-2kg	Alphabiosciences	2kg	EUR 313.2

SIM MEDIUM
S19-110-500g	Alphabiosciences	500 g	EUR 128.4

SOB MEDIUM
S19-124-10kg	Alphabiosciences	10 kg	EUR 1158

SOB MEDIUM
S19-124-2kg	Alphabiosciences	2kg	EUR 298.8

SOB MEDIUM
S19-124-500g	Alphabiosciences	500 g	EUR 124.8

Ec Medium 500gm
231430	Scientific Laboratory Supplies	EACH	EUR 176.4

TCBS Medium
MED1188	Scientific Laboratory Supplies	PK10	EUR 9.6

M9CA Medium
SD7025	Bio Basic	250g	EUR 86.1

Sob Medium 500gm
244310	Scientific Laboratory Supplies	EACH	EUR 261.6

Diss Media Diss (USP/EP) Sim Gastric Fluid w/o enzyme
DB12-10	Scientific Laboratory Supplies	10L	EUR 228

DNase Medium
MED1196	Scientific Laboratory Supplies	PK10	EUR 10.8

TYGPN Medium
SD7032	Bio Basic	250g	EUR 84.01

Heller's Medium
CP014-010	GenDepot	10X1L	EUR 118.8

Heller's Medium
CP014-500	GenDepot	50L	EUR 151.2

Diss Media Diss (USP/EP) Sim Intest Fluid w/o enzyme - 1L
DB13-121	Scientific Laboratory Supplies	PK12	EUR 294

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001	Allele Biotech	1 liter	Ask for price

Mounting Medium
4300	Virostat	3 ml	EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

Advanced Medium
C0003-04	Addexbio	RT 500 mL Bottle	EUR 123.6

COLIFORM MEDIUM
C03-127-10kg	Alphabiosciences	10 kg	EUR 1588.8

COLIFORM MEDIUM
C03-127-2kg	Alphabiosciences	2kg	EUR 392.4

COLIFORM MEDIUM
C03-127-500g	Alphabiosciences	500 g	EUR 150

Hoagland's Medium
CP015-010	GenDepot	10X1L	EUR 118.8

Hoagland's Medium
CP015-500	GenDepot	50L	EUR 151.2

Diss Media Diss (USP/EP) Sim Gastric Fluid w/o enzyme 7L
DB12-07	Scientific Laboratory Supplies	EACH	EUR 175.2

Diss Media Diss (USP/EP) Sim Gastric Fluid w/o enzyme - 1L
DB12-121	Scientific Laboratory Supplies	PK12	EUR 294

Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002	Allele Biotech	1 liter	Ask for price

RPMI 1640 Medium
abx098879-500ml	Abbexa	500 ml	EUR 210

A-1 MEDIUM
A01-116-10kg	Alphabiosciences	10 kg	EUR 1240.8

A-1 MEDIUM
A01-116-2Kg	Alphabiosciences	2 Kg	EUR 316.8

A-1 MEDIUM
A01-116-500g	Alphabiosciences	500 g	EUR 129.6

MCDB 131 Medium
CM034-050	GenDepot	500ml	EUR 102

MCDB 131 Medium
CM034-300	GenDepot	6x500ml	EUR 198

MCDB 131 Medium
CM034-310	GenDepot	10x500ml	EUR 262.8

MCDB 131 Medium
CM034-320	GenDepot	20x500ml	EUR 408

MCDB 131 Medium
CM034-350	GenDepot	50x500ml	EUR 702

Medium 199/BCDB 105
C0012-02	Addexbio	RT 500 mL Bottle	EUR 181.2

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
ABP-MED-10004	Allele Biotech	1 liter	Ask for price

Fluorguard Antifade Mounting Medium
F001	Insitus Biotechnologies	1 ml	EUR 103.2

Isp Medium 2 500gm
277010	Scientific Laboratory Supplies	EACH	EUR 356.4

Isp Medium 4 500gm
277210	Scientific Laboratory Supplies	EACH	EUR 340.8

BPS Medium 1
79259	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with many BPS Reporter Kits including_x000D_ • 60612: AP1 Reporter Kit (JNK Pathway)_x000D_ • 60514: ARE Reporter Kit (Nrf2 Antioxidant Pathway)_x000D_ • 60611: CRE/CREB Reporter Kit (cAMP/PKA Signaling Pathway)_x000D_ • 60614: NF-κB Reporter Kit (NF-κB Signaling Pathway)_x000D_ • 60509: Notch1/CSL Reporter Kit (Notch Signaling Pathway)_x000D_ • 60654: SBE Reporter Kit (TGFβ/SMAD signaling pathway)_x000D_ • 60511: SRE Reporter Kit (MAPK/ERK Signaling Pathway)_x000D_ • 60500: TCF/LEF Reporter Kit (Wnt Signaling Pathway)

BPS Medium 7
79260	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with select BPS reporter kits.

BPS Medium 8
79385	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with the CSF1R/SRE Reporter Kit (BPS Bioscience, #79379).

Rees Ecker Fluid
RRSP664-D	Atom Scientific	500ml	EUR 7.92

Thaw Medium 8
79652-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645