BACKGROUND
Visualization and molecular profiling data analysis together with biological tissue can provide new mechanistic insight into the biological function. At present, it is possible to visualize high-throughput of data over the network layout has been determined, but they are not always suitable for the tasks of data analysis are given. A network layout based simultaneously on the network structure and associated multidimensional data may be advantageous for data visualization and analysis in some cases.
RESULTS
We develop Cytoscape application, which allows to build a biological network layout based on data from the molecular profile is imported as the values of the attribute node. Dedal is 3 Cytoscape application, which uses an algorithm of linear and non-linear dimension reduction for network layout generate data-driven based on the multidimensional data (usually the expression of genes). tools Dedal some data pre-processing and post-processing layout step as a continuous morphing between two arbitrary network layout and align the network layout with respect to each other by rotating and mirroring.
The combination of all of these functions facilitate the creation of the network layout insight which represents both structural network features and patterns of correlations in multivariate data. We demonstrate the added value of the application Dedal in several practical applications, including tissue samples large protein-protein interactions.
DeDaL: Cytoscape 3 app for producing and morphing data-driven and structure-driven network layouts.
Detection of miRNA regulatory effects on triple negative breast cancer transcriptome.
Identify key microRNAs (miRNAs) contributing to the origin and development of certain diseases is the focus of much recent research. We introduce here the rank-based algorithms for detecting miRNA regulatory activity in cancer tissue samples revealed that combining the measurements of gene and the expression levels of miRNA and target sequence-based prediction. This method is designed to detect modest but coordinated changes in expression of the target gene-sequence predicted based. We apply an algorithm to a cohort of 129 breast tumor and the healthy tissue and shown to be effective in identifying functional miRNAs may be involved in disease.
RMI1 Antibody
1-CSB-PA875699LA01HU
Cusabio
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Description: A polyclonal antibody against RMI1. Recognizes RMI1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000
RMI1 Antibody
1-CSB-PA019754GA01HU
Cusabio
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Description: A polyclonal antibody against RMI1. Recognizes RMI1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC
RMI1 Antibody
E94991
EnoGene
100μg
EUR 255
Description: Available in various conjugation types.
RMI1 Antibody
E308764
EnoGene
200ul
EUR 275
Description: Available in various conjugation types.
RMI1 antibody
70R-19909
Fitzgerald
50 ul
EUR 289
Description: Rabbit polyclonal RMI1 antibody
RMI1 antibody
70R-5555
Fitzgerald
50 ug
EUR 467
Description: Rabbit polyclonal RMI1 antibody
RMI1 Antibody
MBS7000931-005mg
MyBiosource
0.05mg
EUR 190
RMI1 Antibody
MBS7000931-01mg
MyBiosource
0.1mg
EUR 270
RMI1 Antibody
MBS7000931-5x01mg
MyBiosource
5x0.1mg
EUR 1205
RMI1 Antibody
MBS8505773-01mg
MyBiosource
0.1mg
EUR 325
RMI1 Antibody
MBS8505773-01mLAF405L
MyBiosource
0.1mL(AF405L)
EUR 565
RMI1 Antibody
MBS8505773-01mLAF405S
MyBiosource
0.1mL(AF405S)
EUR 565
RMI1 Antibody
MBS8505773-01mLAF610
MyBiosource
0.1mL(AF610)
EUR 565
RMI1 Antibody
MBS8505773-01mLAF635
MyBiosource
0.1mL(AF635)
EUR 565
RMI1 Antibody
MBS9611475-01mL
MyBiosource
0.1mL
EUR 260
RMI1 Antibody
MBS9611475-02mL
MyBiosource
0.2mL
EUR 305
RMI1 Antibody
MBS9611475-5x02mL
MyBiosource
5x0.2mL
EUR 1220
RMI1 (untagged)-Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1)
SC322629
Origene Technologies GmbH
10 µg
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RMI1 (untagged)-Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1)
SC111381
Origene Technologies GmbH
10 µg
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RMI1 (GFP-tagged) - Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1)
RG208030
Origene Technologies GmbH
10 µg
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RMI1 (Myc-DDK-tagged)-Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1)
RC208030
Origene Technologies GmbH
10 µg
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RMI1 Rabbit pAb
A4991-100ul
Abclonal
100 ul
EUR 369.6
RMI1 Rabbit pAb
A4991-200ul
Abclonal
200 ul
EUR 550.8
RMI1 Rabbit pAb
A4991-20ul
Abclonal
20 ul
EUR 219.6
RMI1 Rabbit pAb
A4991-50ul
Abclonal
50 ul
EUR 267.6
RMI1 cDNA Clone
MBS1276534-001mgPlasmid02mLGlycerolStock
MyBiosource
0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200
RMI1 cDNA Clone
MBS1276534-5x001mgPlasmid5x02mLGlycerolStock
MyBiosource
5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855
RMI1 Rabbit pAb
A4991
Abclonal
100μL
EUR 262.15
RMI1 siRNA (Human)
MBS8214817-15nmol
MyBiosource
15nmol
EUR 405
RMI1 siRNA (Human)
MBS8214817-30nmol
MyBiosource
30nmol
EUR 565
RMI1 siRNA (Human)
MBS8214817-5x30nmol
MyBiosource
5x30nmol
EUR 2450
RMI1 siRNA (Mouse)
MBS8208894-15nmol
MyBiosource
15nmol
EUR 405
RMI1 siRNA (Mouse)
MBS8208894-30nmol
MyBiosource
30nmol
EUR 565
RMI1 siRNA (Mouse)
MBS8208894-5x30nmol
MyBiosource
5x30nmol
EUR 2450
anti- RMI1 antibody
FNab07321
FN Test
100µg
EUR 702
Description: Antibody raised against RMI1
RMI1 cloning plasmid
CSB-CL875699HU-10ug
Cusabio
10ug
EUR 279.6
Description: A cloning plasmid for the RMI1 gene.
RMI1 Blocking Peptide
33R-1953
Fitzgerald
100 ug
EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of RMI1 antibody, catalog no. 70R-5555
RMI1 Blocking Peptide
DF12724-BP
Affbiotech
1mg
EUR 234
RMI1 Blocking Peptide
MBS9621428-1mg
MyBiosource
1mg
EUR 380
RMI1 Blocking Peptide
MBS9621428-5x1mg
MyBiosource
5x1mg
EUR 1650
RMI1 Polyclonal Antibody
A67578
EpiGentek
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100 µg
20 ul
50 ul
100 ul
RMI1 Polyclonal Antibody
30629
SAB
100ul
EUR 439
RMI1 Polyclonal Antibody
30629-100ul
SAB
100ul
EUR 302.4
RMI1 Polyclonal Antibody
30629-50ul
SAB
50ul
EUR 224.4
RMI1 Polyclonal Antibody
E-AB-92626-120uL
Elabscience Biotech
120uL
EUR 320
Description: Unconjugated
RMI1 Polyclonal Antibody
E-AB-92626-200uL
Elabscience Biotech
200uL
EUR 530
Description: Unconjugated
RMI1 Polyclonal Antibody
E-AB-92626-60uL
Elabscience Biotech
60uL
EUR 200
Description: Unconjugated
RMI1 Polyclonal Antibody
E-AB-92626-each
Elabscience Biotech
each
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Description: Unconjugated
RMI1 Polyclonal Antibody
MBS129174-002mL
MyBiosource
0.02mL
EUR 200
RMI1 Polyclonal Antibody
MBS129174-005mL
MyBiosource
0.05mL
EUR 255
RMI1 Polyclonal Antibody
MBS129174-01mL
MyBiosource
0.1mL
EUR 345
RMI1 Polyclonal Antibody
MBS129174-02mL
MyBiosource
0.2mL
EUR 545
RMI1 Polyclonal Antibody
MBS129174-5x02mL
MyBiosource
5x0.2mL
EUR 2265
RMI1 Polyclonal Antibody
A72829
EpiGentek
50 μl
EUR 408
RMI1 Polyclonal Antibody
MBS9433760-005mL
MyBiosource
0.05mL
EUR 300
RMI1 Polyclonal Antibody
MBS9433760-01mL
MyBiosource
0.1mL
EUR 390
RMI1 Polyclonal Antibody
MBS9433760-5x01mL
MyBiosource
5x0.1mL
EUR 1610
Rmi1 (untagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 2, (10ug)
MC219739
Origene Technologies GmbH
10 µg
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Rmi1 (untagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 1, (10ug)
MC219740
Origene Technologies GmbH
10 µg
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Rmi1 (Myc-DDK-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 1
MR223479
Origene Technologies GmbH
10 µg
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Rmi1 (Myc-DDK-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 2
MR209446
Origene Technologies GmbH
10 µg
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Rmi1 (GFP-tagged) - Mouse RMI1 RecQ mediated genome instability 1 homolog (S. cerevisiae) (Rmi1) transcript variant 1, (10ug)
MG223479
Origene Technologies GmbH
10 µg
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RMI1 Antibody (N-term)
MBS9201450-008mL
MyBiosource
0.08mL
EUR 210
RMI1 Antibody (N-term)
MBS9201450-04mL
MyBiosource
0.4mL
EUR 430
RMI1 Antibody (N-term)
MBS9201450-5x04mL
MyBiosource
5x0.4mL
EUR 1910
Mouse RMI1 shRNA Plasmid
20-abx978212
Abbexa
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Human RMI1 shRNA Plasmid
20-abx962721
Abbexa
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Human RMI1 Protein Lysate
MBS8418638-002mg
MyBiosource
0.02mg
EUR 365
Human RMI1 Protein Lysate
MBS8418638-5x002mg
MyBiosource
5x0.02mg
EUR 1410
RMI1 Antibody, HRP conjugated
1-CSB-PA875699LB01HU
Cusabio
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Description: A polyclonal antibody against RMI1. Recognizes RMI1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
RMI1 Antibody; HRP conjugated
MBS7001280-005mg
MyBiosource
0.05mg
EUR 190
RMI1 Antibody; HRP conjugated
MBS7001280-01mg
MyBiosource
0.1mg
EUR 270
RMI1 Antibody; HRP conjugated
MBS7001280-5x01mg
MyBiosource
5x0.1mg
EUR 1205
RMI1 Antibody, FITC conjugated
1-CSB-PA875699LC01HU
Cusabio
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Description: A polyclonal antibody against RMI1. Recognizes RMI1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
RMI1 Antibody; FITC conjugated
MBS7006670-005mg
MyBiosource
0.05mg
EUR 190
RMI1 Antibody; FITC conjugated
MBS7006670-01mg
MyBiosource
0.1mg
EUR 270
RMI1 Antibody; FITC conjugated
MBS7006670-5x01mg
MyBiosource
5x0.1mg
EUR 1205
RMI1 Recombinant Protein (Human)
RP026503
ABM
100 ug
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RMI1 Recombinant Protein (Mouse)
RP168335
ABM
100 ug
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RMI1 Recombinant Protein (Mouse)
RP168338
ABM
100 ug
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Rmi1 (GFP-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S
MG209446
Origene Technologies GmbH
10 µg
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RMI1 Antibody, Biotin conjugated
1-CSB-PA875699LD01HU
Cusabio
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Description: A polyclonal antibody against RMI1. Recognizes RMI1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
RMI1 Antibody; Biotin conjugated
MBS7000698-005mg
MyBiosource
0.05mg
EUR 190
RMI1 Antibody; Biotin conjugated
MBS7000698-01mg
MyBiosource
0.1mg
EUR 270
RMI1 Antibody; Biotin conjugated
MBS7000698-5x01mg
MyBiosource
5x0.1mg
EUR 1205
RMI1 Polyclonal Conjugated Antibody
C30629
SAB
100ul
EUR 476.4
RMI1 Polyclonal Conjugated Antibody
MBS9441805-01mLAF350
MyBiosource
0.1mL(AF350)
EUR 480
RMI1 Polyclonal Conjugated Antibody
MBS9441805-01mLAF405
MyBiosource
0.1mL(AF405)
EUR 480
RMI1 Polyclonal Conjugated Antibody
MBS9441805-01mLAF488
MyBiosource
0.1mL(AF488)
EUR 480
RMI1 Polyclonal Conjugated Antibody
MBS9441805-01mLAF555
MyBiosource
0.1mL(AF555)
EUR 480
RMI1 Polyclonal Conjugated Antibody
MBS9441805-01mLBiotin
MyBiosource
0.1mL(Biotin)
EUR 480
Lenti ORF particles, RMI1 (mGFP-tagged) - Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1), 200ul, >10^7 TU/mL
RC208030L2V
Origene Technologies GmbH
200 µl
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Lenti ORF particles, RMI1 (mGFP-tagged) - Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1), 200ul, >10^7 TU/mL
RC208030L4V
Origene Technologies GmbH
200 µl
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Lenti ORF clone of Rmi1 (mGFP-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 1
MR223479L4
Origene Technologies GmbH
10 µg
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Lenti ORF clone of Rmi1 (mGFP-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 2
MR209446L4
Origene Technologies GmbH
10 µg
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Lenti ORF particles, Rmi1 (GFP-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 1, 20
MR223479L4V
Origene Technologies GmbH
200 µl
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Lenti ORF particles, Rmi1 (GFP-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 2, 20
MR209446L4V
Origene Technologies GmbH
200 µl
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Lenti ORF particles, Rmi1 (Myc-DDK-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 1
MR223479L3V
Origene Technologies GmbH
200 µl
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Lenti ORF particles, Rmi1 (Myc-DDK-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 2
MR209446L3V
Origene Technologies GmbH
200 µl
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RMI1 ORF Vector (Human) (pORF)
ORF008835
ABM
1.0 ug DNA
EUR 114
Rmi1 ORF Vector (Mouse) (pORF)
ORF056113
ABM
1.0 ug DNA
EUR 607.2
Rmi1 ORF Vector (Mouse) (pORF)
ORF056114
ABM
1.0 ug DNA
EUR 607.2
BLAP75 (RMI1) (NM_024945) Human Recombinant Protein
PROTQ9H9A7
BosterBio
20ug
EUR 1249
Description: Purified recombinant protein of Homo sapiens RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1)
Lenti ORF particles, RMI1 (Myc-DDK tagged) - Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1), 200ul, >10^7 TU/mL
RC208030L1V
Origene Technologies GmbH
200 µl
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Lenti ORF particles, RMI1 (Myc-DDK tagged) - Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1), 200ul, >10^7 TU/mL
RC208030L3V
Origene Technologies GmbH
200 µl
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Lenti ORF clone of Rmi1 (Myc-DDK-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 1
MR223479L3
Origene Technologies GmbH
10 µg
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Lenti ORF clone of Rmi1 (Myc-DDK-tagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 2
MR209446L3
Origene Technologies GmbH
10 µg
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Human RMI1 Protein Lysate 20ug
IHURMI1PLLY20UG
Innovative research
each
EUR 213
Description: Human RMI1 Protein Lysate 20ug
RMI1 Peptide - N-terminal region
MBS3233127-01mg
MyBiosource
0.1mg
EUR 180
RMI1 Peptide - N-terminal region
MBS3233127-5x01mg
MyBiosource
5x0.1mg
EUR 730
RMI1 antibody - N-terminal region
MBS3208164-01mL
MyBiosource
0.1mL
EUR 455
RMI1 antibody - N-terminal region
MBS3208164-5x01mL
MyBiosource
5x0.1mL
EUR 1995
Recombinant Human RMI1, GST-tagged
RMI1-2319H
Creative BioMart
25ug
EUR 255.2
Description: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
RMI1 Over-expression Lysate Product
GWB-28B18E
GenWay Biotech
0.1 mg
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RMI1 Polyclonal Antibody, HRP Conjugated
A67579
EpiGentek
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RMI1 Polyclonal Antibody, FITC Conjugated
A67580
EpiGentek
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RMI1 Polyclonal Antibody, Biotin Conjugated
A67581
EpiGentek
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BLAP75 (RMI1) (NM_024945) Human Over-expression Lysate
LS080010
BosterBio
100ug
EUR 628
Description: Transient overexpression lysate of RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1)
RMI1 sgRNA CRISPR Lentivector set (Human)
K1827701
ABM
3 x 1.0 ug
EUR 406.8
Rmi1 sgRNA CRISPR Lentivector set (Mouse)
K4476601
ABM
3 x 1.0 ug
EUR 406.8
RMI1 Antibody (N-term) Blocking Peptide
MBS9222072-INQUIRE
MyBiosource
INQUIRE
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RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75)
MBS6012524-02mL
MyBiosource
0.2(mL
EUR 695
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75)
MBS6012524-5x02mL
MyBiosource
5x0.2mL
EUR 2975
Rmi1 3'UTR GFP Stable Cell Line
TU167918
ABM
1.0 ml
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Rmi1 3'UTR GFP Stable Cell Line
TU269470
ABM
1.0 ml
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RMI1 3'UTR GFP Stable Cell Line
TU069953
ABM
1.0 ml
EUR 1672.8
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (AP)
MBS6342335-02mL
MyBiosource
0.2mL
EUR 980
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (AP)
MBS6342335-5x02mL
MyBiosource
5x0.2mL
EUR 4250
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (PE)
MBS6342345-02mL
MyBiosource
0.2mL
EUR 980
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (PE)
MBS6342345-5x02mL
MyBiosource
5x0.2mL
EUR 4250
Lenti ORF clone of Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1), mGFP tagged
RC208030L2
Origene Technologies GmbH
10 µg
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Lenti ORF clone of Human RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1), mGFP tagged
RC208030L4
Origene Technologies GmbH
10 µg
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RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (APC)
MBS6342336-02mL
MyBiosource
0.2mL
EUR 980
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (APC)
MBS6342336-5x02mL
MyBiosource
5x0.2mL
EUR 4250
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (Biotin)
MBS6342337-02mL
MyBiosource
0.2mL
EUR 980
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (Biotin)
MBS6342337-5x02mL
MyBiosource
5x0.2mL
EUR 4250
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (FITC)
MBS6342338-02mL
MyBiosource
0.2mL
EUR 980
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (FITC)
MBS6342338-5x02mL
MyBiosource
5x0.2mL
EUR 4250
RMI1 Protein Vector (Human) (pPM-C-HA)
PV035339
ABM
500 ng
EUR 394.8
RMI1 Protein Vector (Mouse) (pPM-C-HA)
PV224452
ABM
500 ng
EUR 723.6
RMI1 Protein Vector (Mouse) (pPM-C-HA)
PV224456
ABM
500 ng
EUR 723.6
RMI1 3'UTR Luciferase Stable Cell Line
TU019953
ABM
1.0 ml
EUR 1672.8
Rmi1 3'UTR Luciferase Stable Cell Line
TU117918
ABM
1.0 ml
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RMI1 Protein Vector (Human) (pPB-C-His)
PV035337
ABM
500 ng
EUR 394.8
RMI1 Protein Vector (Human) (pPB-N-His)
PV035338
ABM
500 ng
EUR 394.8
RMI1 Protein Vector (Human) (pPM-C-His)
PV035340
ABM
500 ng
EUR 394.8
RMI1 Protein Vector (Mouse) (pPB-C-His)
PV224450
ABM
500 ng
EUR 723.6
RMI1 Protein Vector (Mouse) (pPB-N-His)
PV224451
ABM
500 ng
EUR 723.6
RMI1 Protein Vector (Mouse) (pPM-C-His)
PV224453
ABM
500 ng
EUR 723.6
RMI1 Protein Vector (Mouse) (pPB-C-His)
PV224454
ABM
500 ng
EUR 723.6
RMI1 Protein Vector (Mouse) (pPB-N-His)
PV224455
ABM
500 ng
EUR 723.6
This observation has been validated using breast cancer datasets publicly available independent of The Cancer Genome Atlas. We focus on triple negative breast cancer subtypes to highlight potentially relevant miRNAs in these tumor subtypes. For those miRNAs were identified as potential regulators, we characterize the function of target genes affected by enrichment analysis. In two independent datasets, targets affected are not always the same, but displays a similar enriched categories, including breast cancer-related processes such as cell substrate adherens junction, regulation of cell migration, nuclear pore complex and the integrin pathway.