Multiplex Cytokine Elisa Kit

Product Overview

The Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-4, IL-6, IL-10, IFNγ).

Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

Assay Ranges

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

Reagents include:

Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;_x000D_Detection Mix: Lyophilized, biotinylated antibodies in a buffered protein solution with preservatives. Reconstitute to 6 mL with Ultra Pure Lab Water;_x000D_Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide;_x000D_Substrate B+: Liquid, 3 mL/vial of stabilized signal enhancer;_x000D_Plate Seals (3): Adhesive strips

Product Overview

The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4.83 hours.

The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing.

Reagents include:

Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;_x000D_Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered solution with preservatives;_x000D_Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide;_x000D_Substrate B+: Liquid, 3 mL/vial of stabilized signal enhancer;_x000D_Plate Seals (3): Adhesive strips